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PMID: 1695894 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

The ompA 5' untranslated RNA segment functions in Escherichia coli as a growth-rate-regulated mRNA stabilizer whose activity is unrelated to translational efficiency.

Journal of bacteriology ·Vol. 172 ·No. 8 ·1990-08-00 ·Pages 4472-81

Emory SA, Belasco JG

Abstract

The 5' untranslated region (UTR) of the long-lived Escherichia coli ompA message can function in vivo as an mRNA stabilizer. Substitution of this ompA mRNA segment for the corresponding segment of the labile bla gene transcripts prolongs their lifetime by a factor of 6. We show here that the function of this ompA mRNA stabilizer requires the presence of a 115-nucleotide ompA RNA segment that lies upstream of the ribosome-binding site. Although deletion of this segment reduced the half-life of the ompA transcript by a factor of 5, its absence had almost no effect on the translational efficiency of ompA mRNA. Like the ompA transcript, but unlike bla mRNA, hybrid ompA-bla messages containing the complete ompA 5' UTR were significantly less stable under conditions of slow bacterial growth. We conclude that the stabilizing activity of the ompA 5' UTR is growth rate regulated and that the mechanism of mRNA stabilization by this RNA segment is not related to the spacing between translating ribosomes.

MeSH Terms
Bacterial Outer Membrane Proteins/genetics Base Sequence Escherichia coli/genetics,growth & development Gene Expression Regulation, Bacterial Kinetics Molecular Sequence Data Plasmids Protein Biosynthesis RNA, Bacterial/genetics,isolation & purification RNA, Messenger/genetics,metabolism Transcription, Genetic
Chemicals
Bacterial Outer Membrane Proteins RNA, Bacterial RNA, Messenger
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Emory S A
Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115.
Belasco J G
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.172.8.4472-4481.1990
Published
1990-08-00
Pages
4472-81
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC213277
Subset
IM
Grants
NIGMS NIH HHS · GM35769 · United States
External Links
PubMed source DOI: 10.1128/jb.172.8.4472-4481.1990 PMC Full Text View journal details
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