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PMID: 3312955 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The functional stability of the lacZ transcript is sensitive towards sequence alterations immediately downstream of the ribosome binding site.

Molecular & general genetics : MGG ·Vol. 209 ·No. 1 ·1987-08-00 ·Pages 179-87

Petersen C

Abstract

Various synthetic DNA sequences were inserted downstream of the fourth codon of the Escherichia coli lacZ gene on plasmids containing a hybrid lacZ-galK operon. Several different sequences, one as short as 10 bp, reduced the functional stability of the lacZ message three- to fourfold, whereas others had little or no effect. Introduction of synthetic sequences into a plasmid containing the intact lac operon resulted in similar reductions of mRNA stability. The sequence alterations also reduced the translational efficiency and transcription through lacZ as monitored by measurements of galactokinase synthesis from the downstream galK gene. There was no correlation between the average translational frequency and the stability of the lacZ message indicating that some of the inserted sequences reduced mRNA stability directly and not as a consequence of their effect on translation. The reduction of transcription through the lacZ gene correlated with the reduction of translation in agreement with current models of transcriptional polarity.

MeSH Terms
Amino Acid Sequence Base Composition Base Sequence Escherichia coli/genetics Genes Genes, Bacterial Lac Operon Nucleic Acid Conformation Plasmids Protein Biosynthesis RNA, Messenger/genetics,metabolism Ribosomes/metabolism Transcription, Genetic
Chemicals
RNA, Messenger
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Petersen C
Institute of Microbiology, University of Copenhagen, Denmark.
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00329856
Published
1987-08-00
Pages
179-87
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00329856
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