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PMID: 3901000 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

DNA sequence of the lactose operon: the lacA gene and the transcriptional termination region.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 19 ·1985-10-00 ·Pages 6414-8

Hediger MA, Johnson DF, Nierlich DP, Zabin I

Abstract

The lac operon of Escherichia coli spans approximately 5300 base pairs and includes the lacZ, lacY, and lacA genes in addition to the operator, promoter, and transcription termination regions. We report here the sequence of the lacA gene and the region distal to it, confirming the sequence of thiogalactoside transacetylase and completing the sequence of the lac operon. The lacA gene is characterized by use of rare codons, suggesting an origin from a plasmid, transposon, or virus gene. UUG is the translation initiation codon. A preliminary examination of 3' end of the lac messenger in the region distal to the lacA gene indicates several endpoints. A predominant one is located at the 3' end of a G + C-rich hairpin structure, which may be involved in termination of transcription or in post-transcriptional processing. An open reading frame of 702 base pairs is present on the complementary strand downstream from lacA.

MeSH Terms
Acetyltransferases/genetics Bacterial Proteins/genetics Base Sequence Codon DNA, Bacterial/genetics Escherichia coli/genetics Genes Genes, Bacterial Nucleic Acid Conformation Operon Recombinant Proteins/genetics Terminator Regions, Genetic
Chemicals
Bacterial Proteins Codon DNA, Bacterial Recombinant Proteins Acetyltransferases galactoside acetyltransferase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Hediger M A
Johnson D F
Nierlich D P
Zabin I
References (37)
37 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.19.6414
Published
1985-10-00
Pages
6414-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC390726
Subset
IM
Grants
NIAID NIH HHS · AI-04181 · United States
Databases
GENBANK
J01636, J01637, K01483, K01793
External Links
PubMed source DOI: 10.1073/pnas.82.19.6414 PMC Full Text View journal details
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