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PMID: 6393056 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The complete nucleotide sequence of the adenylate cyclase gene of Escherichia coli.

Nucleic acids research ·Vol. 12 ·No. 24 ·1984-12-21 ·Pages 9427-40

Aiba H, Mori K, Tanaka M, Ooi T, Roy A, Danchin A

Abstract

The complete nucleotide sequence of the cya gene from E. coli was determined. The gene encodes a polypeptide consisting of 848 amino acid residues with a calculated molecular weight of 97,542. The deduced protein structure reveals that cyclase is comprised of two domains, an amino-terminal region exhibiting catalytic activity and a carboxy-terminal region possibly carrying regulatory function. The frequent appearance of rare codons in the beginning of the gene as well as the sequence duplication in the promoter-initiator region suggest possible regulation(s) at the translational level. An unknown gene (cyaX) which seems to code for a very hydrophobic protein was found following the cya gene. Sequence analysis suggests that the cyax is a part of the cya operon.

MeSH Terms
Adenylyl Cyclases/genetics Amino Acid Sequence Base Sequence Codon Escherichia coli/enzymology,genetics Gene Expression Regulation Genes Genes, Bacterial Operon Protein Conformation
Chemicals
Codon Adenylyl Cyclases
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Aiba H
Mori K
Tanaka M
Ooi T
Roy A
Danchin A
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23 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/12.24.9427
Published
1984-12-21
Pages
9427-40
Language
English
Region
England
NLM ID
0411011
PMCID
PMC320471
Subset
IM
Databases
GENBANK
X01653
External Links
PubMed source DOI: 10.1093/nar/12.24.9427 PMC Full Text View journal details
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