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PMID: 7012833 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Feedback regulation of ribosomal protein gene expression in Escherichia coli: structural homology of ribosomal RNA and ribosomal protein MRNA.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 77 ·No. 12 ·1980-12-00 ·Pages 7084-8

Nomura M, Yates JL, Dean D, Post LE

Abstract

Certain ribosomal proteins (r proteins) in Escherichia coli, such as S4 and S7, function as feedback repressors in the regulation of r-protein synthesis. These proteins inhibit the translation of their own mRNA. The repressor r proteins so far identified are also known to bind specifically to rRNA at an initial stage in ribosome assembly. We have found structural homology between the S7 binding region on 16S rRNA and a region of the mRNA where S7 acts as a translational repressor. Similarly, there is structural homology between one of the reported S4 binding regions on 16S rRNA and the mRNA target site for S4. The observed homology supports the concept that regulation by repressor r proteins is based on competition between rRNA and mRNA for these proteins and that the same structural features and of the r proteins are used in their interactions with both rRNA and mRNA.

MeSH Terms
Bacterial Proteins/genetics Binding Sites Escherichia coli/genetics Feedback Gene Expression Regulation Nucleic Acid Conformation Protein Binding Protein Biosynthesis RNA, Messenger/genetics RNA, Ribosomal/genetics Ribosomal Proteins/genetics
Chemicals
Bacterial Proteins RNA, Messenger RNA, Ribosomal Ribosomal Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Nomura M
Yates J L
Dean D
Post L E
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27 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.77.12.7084
Published
1980-12-00
Pages
7084-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC350445
Subset
IM
Grants
NIGMS NIH HHS · GM-20427 · United States
NIGMS NIH HHS · GMO 7553 · United States
External Links
PubMed source DOI: 10.1073/pnas.77.12.7084 PMC Full Text View journal details
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