Max-Planck-Institut für Molekulare Genetik, Abteilung Wittmann, Berlin-Dahlem, GFR.

Nucleic acids research ·Vol. 6 ·No. 5 ·1979-00-00 ·Pages 1775-90

Zwieb C, Brimacombe R

Abstract

It is well established that when E. coli 30S ribosomal subunits are irradiated with ultraviolet light under mild conditions a specific cross-link is formed between protein S7 and the 16S RNA. Methodology is presented for the analysis of the single nucleotide residue concerned in this cross-link. Firstly, the identity of the ribonuclease T1 octanucleotide attached to S7 is confirmed by a new method, which involves isolation and analysis of S7-polynucleotide complexes containing 30 -- 40 nucleotides. Secondly, the isolated S7-octanucleotide complex is digested successively with ribonuclease A, proteinase K and ribonuclease T2, and the nucleotides liberated are identified. The results show unambiguously that uridine residue number 1239 in the 16S RNA sequence is cross-linked to protein S7.

MeSH Terms

Base Sequence Escherichia coli/analysis Molecular Weight Nucleoproteins/analysis Oligoribonucleotides/analysis Protein Binding RNA, Ribosomal/radiation effects Ribonucleoproteins/analysis Ribosomal Proteins/radiation effects Ribosomes/analysis Ultraviolet Rays

Chemicals

Nucleoproteins Oligoribonucleotides RNA, Ribosomal Ribonucleoproteins Ribosomal Proteins

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Zwieb C

Brimacombe R

References (24)

24 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/6.5.1775

Published

1979-00-00

Pages

1775-90

Language

English

Region

England

NLM ID

0411011

PMCID

PMC327810

Subset

External Links

PubMed source DOI: 10.1093/nar/6.5.1775 PMC Full Text View journal details

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