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PMID: 1372602 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Characterization of the Lactococcus lactis lactose operon promoter: contribution of flanking sequences and LacR repressor to promoter activity.

Journal of bacteriology ·Vol. 174 ·No. 7 ·1992-04-00 ·Pages 2273-80

van Rooijen RJ, Gasson MJ, de Vos WM

Abstract

We determined the location, activity, and regulation of the promoter of the Lactococcus lactis 8-kb lactose operon (lacABCDFEGX), which encodes the enzymes of the lactose phosphotransferase system and the tagatose 6-phosphate pathway. The lac promoter sequence corresponds closely to the consensus promoter described for gram-positive bacteria and is located in a back-to-back configuration with the promoter of the divergently transcribed lacR gene, which encodes the LacR repressor. The transcription start sites used under induced (lactose) and noninduced (glucose) conditions were determined. The minimal promoter region that could be isolated on a single restriction fragment included sequences ranging from -75 to +42. The effect of the presence of flanking sequences and the lacR gene on promoter activity and regulation was studied in Escherichia coli and L. lactis strains by using transcriptional fusions with promoterless chloramphenicol acetyltransferase reporter genes. The results showed that transcriptional regulation of the lac operon is mediated by the interaction between the LacR repressor, the lac promoter, and sequences in the noncoding region between the lacR and lacA genes. Sequences flanking the minimal promoter region appeared to enhance lac promoter activity much more in L. lactis (5- to 38-fold) than in E. coli (1.3- to 5-fold).

Related Genes
deo lacA lacR
MeSH Terms
Base Sequence Chromosome Mapping DNA, Bacterial/genetics Escherichia coli/genetics Gene Expression Regulation, Bacterial Hydrogen Bonding Lac Operon Lactococcus lactis/genetics Molecular Sequence Data Nucleic Acid Conformation Promoter Regions, Genetic RNA, Bacterial/ultrastructure RNA, Messenger/ultrastructure Repressor Proteins/genetics Restriction Mapping Sequence Homology, Nucleic Acid Transcription, Genetic
Chemicals
DNA, Bacterial RNA, Bacterial RNA, Messenger Repressor Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
van Rooijen R J
Department of Biophysical Chemistry, Netherlands Institute for Dairy Research (NIZO), Ede.
Gasson M J
de Vos W M
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.174.7.2273-2280.1992
Published
1992-04-00
Pages
2273-80
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC205848
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.174.7.2273-2280.1992 PMC Full Text View journal details
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