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PMID: 2996425 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Construction of cloning, promoter-screening, and terminator-screening shuttle vectors for Bacillus subtilis and Streptococcus lactis.

Applied and environmental microbiology ·Vol. 50 ·No. 2 ·1985-08-00 ·Pages 540-2

van der Vossen JM, Kok J, Venema G

Abstract

Shuttle vectors have been constructed which are suitable both for the selection of regulatory sequences and for gene cloning in Bacillus subtilis and Streptococcus lactis. The promoter screening vectors contain a promoterless chloramphenicol acetyltransferase gene; the insertion of suitable DNA fragments upstream of the gene restored the enzyme activity. With a related set of vectors, transcription termination signals can be selected.

MeSH Terms
Acetyltransferases/genetics Bacillus subtilis/genetics Chloramphenicol O-Acetyltransferase Cloning, Molecular DNA Restriction Enzymes Genes, Bacterial Genes, Regulator Genetic Vectors Lactococcus lactis/genetics Promoter Regions, Genetic Terminator Regions, Genetic
Chemicals
Acetyltransferases Chloramphenicol O-Acetyltransferase DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
van der Vossen J M
Kok J
Venema G
References (21)
21 references, click to expand
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Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
0099-2240
DOI
10.1128/aem.50.2.540-542.1985
Published
1985-08-00
Pages
540-2
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC238658
Subset
IM
External Links
PubMed source DOI: 10.1128/aem.50.2.540-542.1985 PMC Full Text View journal details
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