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PMID: 2120187 Published · ppublish English Journal Article

Spontaneous deletion formation within the beta-galactosidase gene of Lactobacillus bulgaricus.

Journal of bacteriology ·Vol. 172 ·No. 10 ·1990-10-00 ·Pages 5670-6

Mollet B, Delley M

Abstract

To investigate the genetic stability of the dairy organism Lactobacillus bulgaricus, we have analyzed 107 spontaneous mutations of the beta-galactosidase gene of this organism. Ten of these mutations were DNA rearrangements giving rise to different deletions, located predominantly within a small hot spot area. The DNA sequences of the different deletion junctions have been determined. The analysis showed that the deletions can be divided into two classes, depending on the presence of short direct-repeat sequences at the deletion endpoints and on the length of the deleted sequences. Possible mechanisms of these deletion formations and the involvement of inverted-repeat sequences that may enhance slipped DNA mispairing are discussed.

MeSH Terms
Base Sequence Chromosome Deletion DNA, Bacterial/genetics Genes, Bacterial Lactobacillus/enzymology,genetics Models, Genetic Molecular Sequence Data Mutation Nucleic Acid Conformation Oligonucleotide Probes Plasmids Polymerase Chain Reaction Repetitive Sequences, Nucleic Acid Restriction Mapping beta-Galactosidase/genetics
Chemicals
DNA, Bacterial Oligonucleotide Probes beta-Galactosidase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Mollet B
Nestlé Research Centre, Nestec Ltd., Lausanne, Switzerland.
Delley M
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43 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.172.10.5670-5676.1990
Published
1990-10-00
Pages
5670-6
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC526881
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.172.10.5670-5676.1990 PMC Full Text View journal details
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