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PMID: 2546037 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Transcriptional occlusion of transposon targets.

Molecular & general genetics : MGG ·Vol. 216 ·No. 2-3 ·1989-04-00 ·Pages 204-9

Casadesus J, Roth JR

Abstract

In Salmonella typhimurium, insertion of transposons Tn5, Tn10 and bacteriophage Mu is inhibited by transcription of some target sequences. The transcription effects on Tn5 are large when the lac operon is a target but are limited to a slight effect on the hisG gene of the his operon. The Tn10 element shows target occlusion in both operons. Phage Mu has been shown previously to be inhibited for insertion into the lac operon. In the his operon Mu is only inhibited for insertion into the hisG gene. The variability of the inhibition effect from one sequence to another suggests site or regional specificity for transcription effects. Reducing the probability of insertion into transcribed sequences may be of selective importance to transposons since it reduces the risk of killing the host while maintaining the ability to transpose.

MeSH Terms
DNA Transposable Elements Escherichia coli/genetics Genes, Bacterial Histidine/genetics Lac Operon Salmonella typhimurium/genetics Transcription, Genetic
Chemicals
DNA Transposable Elements Histidine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Casadesus J
Department of Biology, University of Utah, Salt Lake City 84122.
Roth J R
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00334357
Published
1989-04-00
Pages
204-9
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Grants
NIGMS NIH HHS · GM 27068 · United States
External Links
PubMed source DOI: 10.1007/BF00334357
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