Genetic separation of hypoxanthine and guanine-xanthine phosphoribosyltransferase activities by deletion mutations in Salmonella typhimurium.

Journal of bacteriology ·Vol. 112 ·No. 2 ·1972-11-00 ·Pages 910-6

Gots JS, Benson CE, Shumas SR

Abstract

Certain proAB deletion mutants of Salmonella typhimurium were found to be simultaneously deleted in a gene required for the utilization of guanine and xanthine (designated gxu). These mutants were resistant to 8-azaguanine and when carrying an additional pur mutation were unable to use guanine or xanthine as a purine source. The defect was correlated with deficiencies in the uptake and phosphoribosyltransferase activities for guanine and xanthine. Hypoxanthine and adenine activities were unaltered. The deficiency was restored to normal by transduction to pro(+) and in F' merodiploids.

MeSH Terms

Adenine/metabolism Azaguanine/pharmacology Carbon Isotopes Cell-Free System Chromosome Mapping Culture Media Drug Resistance, Microbial Genes, Regulator Genetics, Microbial Guanine/metabolism Guanine Nucleotides Hypoxanthines/metabolism Mutation Oxidoreductases/metabolism Pentosyltransferases/metabolism Salmonella typhimurium/drug effects,enzymology,growth & development,metabolism Xanthines/metabolism

Chemicals

Carbon Isotopes Culture Media Guanine Nucleotides Hypoxanthines Xanthines Guanine Oxidoreductases Pentosyltransferases Adenine Azaguanine

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Gots J S

Benson C E

Shumas S R

References (25)

25 references, click to expand

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.112.2.910-916.1972

Published

1972-11-00

Pages

910-6

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC251503

Subset

External Links

PubMed source DOI: 10.1128/jb.112.2.910-916.1972 PMC Full Text View journal details

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Genetic separation of hypoxanthine and guanine-xanthine phosphoribosyltransferase activities by deletion mutations in Salmonella typhimurium.

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