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PMID: 8654379 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A long-range pseudoknot is required for activity of the Neurospora VS ribozyme.

The EMBO journal ·Vol. 15 ·No. 11 ·1996-06-03 ·Pages 2820-5

Rastogi T, Beattie TL, Olive JE, Collins RA

Abstract

Four small RNA self-cleaving domains, the hammerhead, hairpin, hepatitis delta virus and Neurospora VS ribozymes, have been identified previously in naturally occurring RNAs. The secondary structures of these ribozymes are reasonably well understood, but little is known about long-range interactions that form the catalytically active tertiary conformations. Our previous work, which identified several secondary structure elements of the VS ribozyme, also showed that many additional bases were protected by magnesium-dependent interactions, implying that several tertiary contacts remained to be identified. Here we have used site-directed mutagenesis and chemical modification to characterize the first long-range interaction identified in VS RNA. This interaction contains a 3 bp pseudoknot helix that is required for tertiary folding and self-cleavage activity of the VS ribozyme.

MeSH Terms
Diethyl Pyrocarbonate/chemistry Magnesium/chemistry Neurospora/enzymology Nucleic Acid Conformation RNA, Catalytic/chemistry RNA, Fungal/chemistry,ultrastructure Structure-Activity Relationship
Chemicals
RNA, Catalytic RNA, Fungal Magnesium Diethyl Pyrocarbonate
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Rastogi T
Department of Molecular and Medical Genetics, University of Toronto, Ontario, Canada.
Beattie T L
Olive J E
Collins R A
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34 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1996-06-03
Pages
2820-5
Language
English
Region
England
NLM ID
8208664
PMCID
PMC450219
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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