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PMID: 8371972 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Assessment of disparate structural features in three models of the hepatitis delta virus ribozyme.

Nucleic acids research ·Vol. 21 ·No. 17 ·1993-08-25 ·Pages 3959-65

Perrotta AT, Been MD

Abstract

Three models for the secondary structure of the hepatitis delta virus (HDV) antigenomic self-cleaving RNA element were tested by site-directed mutagenesis. Two models in which bases 5' to the cleavage site are paired with sequence at the 3' end of the element were both inconsistent with the data from the mutagenesis. Specifically, mutations in the 3' sequence which decrease self-cleavage activity could not be compensated by base changes in the 5' sequence as predicted by these models. The evidence was consistent with a third model in which the 3' end pairs with a portion of a loop within the ribozyme sequence to generate a pseudoknot structure. This same pairing was also required to generate higher rates of cleavage in trans with a 15-mer ribozyme, thus ruling out a proposed hammerhead-like 'axehead' model for the HDV ribozyme.

MeSH Terms
Base Sequence DNA, Viral Hepatitis Delta Virus/genetics Kinetics Molecular Sequence Data Mutagenesis, Site-Directed Nucleic Acid Conformation RNA, Catalytic/chemistry,metabolism RNA, Viral/chemistry,metabolism
Chemicals
DNA, Viral RNA, Catalytic RNA, Viral
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Perrotta A T
Department of Biochemistry, Duke University Medical Center, Durham, NC 27710.
Been M D
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/21.17.3959
Published
1993-08-25
Pages
3959-65
Language
English
Region
England
NLM ID
0411011
PMCID
PMC309977
Subset
IM
Grants
NIGMS NIH HHS · GM-40689 · United States
External Links
PubMed source DOI: 10.1093/nar/21.17.3959 PMC Full Text View journal details
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