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PMID: 2446263 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Review

Probing the structure of RNAs in solution.

Nucleic acids research ·Vol. 15 ·No. 22 ·1987-11-25 ·Pages 9109-28

Ehresmann C, Baudin F, Mougel M, Romby P, Ebel JP, Ehresmann B

Abstract

During these last years, a powerful methodology has been developed to study the secondary and tertiary structure of RNA molecules either free or engaged in complex with proteins. This method allows to test the reactivity of every nucleotide towards chemical or enzymatic probes. The detection of the modified nucleotides and RNase cleavages can be conducted by two different paths which are oriented both by the length of the studied RNA and by the nature of the probes used. The first one uses end-labeled RNA molecule and allows to detect only scissions in the RNA chain. The second approach is based on primer extension by reverse transcriptase and detects stops of transcription at modified or cleaved nucleotides. The synthesized cDNA fragments are then sized by electrophoresis on polyacrylamide:urea gels. In this paper, the various structure probes used so far are described, and their utilization is discussed.

MeSH Terms
Base Sequence DNA/analysis Molecular Sequence Data Nucleic Acid Conformation RNA/genetics Ribonucleases
Chemicals
RNA DNA Ribonucleases
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Ehresmann C
Laboratoire de Biochimie, Institut de Biologie Moléculaire et Cellulaire du CNRS, Strasbourg, France.
Baudin F
Mougel M
Romby P
Ebel J P
Ehresmann B
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/15.22.9109
Published
1987-11-25
Pages
9109-28
Language
English
Region
England
NLM ID
0411011
PMCID
PMC306456
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/15.22.9109 PMC Full Text View journal details
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