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PMID: 3582366 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Xenopus transcription factor IIIA binds primarily at junctions between double helical stems and internal loops in oocyte 5S RNA.

The EMBO journal ·Vol. 6 ·No. 2 ·1987-02-00 ·Pages 453-60

Christiansen J, Brown RS, Sproat BS, Garrett RA

Abstract

RNases and chemical probes were used to study the accessibility of each nucleotide of 5S RNA in the native and reconstituted 7S particle from Xenopus laevis oocytes. RNase or chemically treated 5S RNA from intact 7S particles was isolated and analysed using an oligodeoxynucleotide primer and reverse transcriptase. The results were superimposed on a cylindrical projection of an RNA double helix and the protection effects were shown to cluster at two regions on the molecular surface. A three-dimensional model is proposed for the 7S particle in which protein-RNA contacts occur mainly in the major groove of 5S RNA.

MeSH Terms
Animals Base Sequence Female Models, Molecular Molecular Weight Nucleic Acid Conformation Oocytes/metabolism Protein Binding RNA, Ribosomal/genetics,metabolism Transcription Factor TFIIIA Transcription Factors/metabolism Xenopus laevis
Chemicals
RNA, Ribosomal Transcription Factor TFIIIA Transcription Factors
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Christiansen J
Brown R S
Sproat B S
Garrett R A
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31 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1987-02-00
Pages
453-60
Language
English
Region
England
NLM ID
8208664
PMCID
PMC553416
Subset
IM
Databases
GENBANK
M21176, M21177, X05089
External Links
PubMed source PMC Full Text View journal details
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