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PMID: 7753865 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

A secondary-structure model for the self-cleaving region of Neurospora VS RNA.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 92 ·No. 10 ·1995-05-09 ·Pages 4686-90

Beattie TL, Olive JE, Collins RA

Abstract

Neurospora VS RNA performs an RNA-mediated self-cleavage reaction whose products contain 2',3'-cyclic phosphate and 5'-hydroxyl termini. This reaction is similar to those of hammerhead, hairpin, and hepatitis delta virus ribozymes; however, VS RNA is not similar in sequence to these other self-cleaving motifs. Here we propose a model for the secondary structure of the self-cleaving region of VS RNA, supported by site-directed mutagenesis and chemical modification structure probing data. The secondary structure of VS RNA is distinct from those of the other naturally occurring RNA self-cleaving domains. In addition to a unique secondary structure, several Mg-dependent interactions occur during the folding of VS RNA into its active tertiary conformation.

MeSH Terms
Cloning, Molecular Electrophoresis, Polyacrylamide Gel Models, Structural Molecular Weight Mutagenesis, Site-Directed Neurospora/metabolism Nucleic Acid Conformation Nucleic Acid Denaturation RNA, Fungal/biosynthesis,chemistry,isolation & purification
Chemicals
RNA, Fungal
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Beattie T L
Canadian Institute for Advanced Research Program in Evolutionary Biology, Department of Molecular and Medical Genetics, University of Toronto, ON.
Olive J E
Collins R A
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.92.10.4686
Published
1995-05-09
Pages
4686-90
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC42009
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.92.10.4686 PMC Full Text View journal details
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