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PMID: 8628277 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Fos-Jun dimerization promotes interaction of the basic region with TFIIE-34 and TFIIF.

Molecular and cellular biology ·Vol. 16 ·No. 5 ·1996-05-00 ·Pages 2110-8

Martin ML, Lieberman PM, Curran T

Abstract

The regulation of RNA polymerase II-mediated transcription involves both direct and indirect interactions among regulatory proteins and the general transcription factors (GTFs) that assemble at TATA-containing promoters. Here we show that the oncogenic transcription factors Fos and Jun make direct physical contacts with three proteins of the basal transcription apparatus, TFIIE-34 (TFIIE-beta), TFIIF-30 (RAP30), and TFIIF-74 (RAP74). The interactions among the activator proteins and these three GTFs were not detected with other transcription factors, including some bZIP protein family members. Both coimmunoprecipitation and protein blotting experiments demonstrated that the interactions were strongly favored by dimerization of Fos and Jun and that they involved the basic region and basic region-proximal domain of both proteins. Mutations within the DNA-binding domains of Fos and Jun abolished binding to GTFs, although the presence of DNA was not required for the association. Surprisingly, only a single basic region in the context of a protein dimer was sufficient for the interaction. Squelching of AP-1-dependent transcription in vitro by an excess of Fos-Jun dimers was relieved by the addition of TFIIE, indicating that it is a direct functional target of Fos and Jun. These results suggest that dimerization induces a conformational alteration in the basic region of Fos and Jun that promotes an association with TFIIE-34 and TFIIF, thus contributing to transcription initiation.

MeSH Terms
Animals Blotting, Western Cell Line Cloning, Molecular Gene Expression Regulation Humans Mutagenesis Plasmids Protein Multimerization Proto-Oncogene Proteins c-fos/biosynthesis,metabolism Proto-Oncogene Proteins c-jun/biosynthesis,metabolism RNA Polymerase II/metabolism Rats Recombinant Fusion Proteins/biosynthesis,metabolism Sequence Tagged Sites Transcription Factor AP-1/metabolism Transcription Factors/metabolism Transcription Factors, TFII Transcription, Genetic
Chemicals
Proto-Oncogene Proteins c-fos Proto-Oncogene Proteins c-jun Recombinant Fusion Proteins Transcription Factor AP-1 Transcription Factors Transcription Factors, TFII transcription factor TFIIE RNA Polymerase II transcription factor TFIIF
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Martin M L
Roche Institute of Molecular Biology, Nutley, New Jersey 07110, USA.
Lieberman P M
Curran T
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.16.5.2110
Published
1996-05-00
Pages
2110-8
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC231198
Subset
IM
Grants
NCI NIH HHS · P30 CA21765 · United States
External Links
PubMed source DOI: 10.1128/MCB.16.5.2110 PMC Full Text View journal details
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