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PMID: 2119000 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Fos and jun cooperate in transcriptional regulation via heterologous activation domains.

Molecular and cellular biology ·Vol. 10 ·No. 10 ·1990-10-00 ·Pages 5532-5

Abate C, Luk D, Gagne E, Roeder RG, Curran T

Abstract

The products of c-fos and c-jun (Fos and Jun) function in gene regulation by interacting with the AP-1 binding site. Here we have examined the contribution of Fos and Jun toward transcriptional activity by using Fos and Jun polypeptides purified from Escherichia coli. Fos contained a transcriptional activation domain as well as a region which exerted a negative influence on transcriptional activity in vitro. Moreover, distinct activation domains in both Fos and Jun functioned cooperatively in transcriptional stimulation. Thus, regulation of gene expression by Fos and Jun results from an integration of several functional domains in a bimolecular complex.

MeSH Terms
Binding Sites Cell-Free System DNA-Binding Proteins/genetics,metabolism Gene Expression Regulation HeLa Cells Humans Macromolecular Substances Proto-Oncogene Proteins/genetics,metabolism Proto-Oncogene Proteins c-fos Proto-Oncogene Proteins c-jun Structure-Activity Relationship Transcription Factors/genetics,metabolism Transcription, Genetic
Chemicals
DNA-Binding Proteins Macromolecular Substances Proto-Oncogene Proteins Proto-Oncogene Proteins c-fos Proto-Oncogene Proteins c-jun Transcription Factors
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Abate C
Department of Molecular Oncology and Virology, Roche Institute of Molecular Biology, Nutley, New Jersey 07110-1199.
Luk D
Gagne E
Roeder R G
Curran T
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33 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.10.10.5532-5535.1990
Published
1990-10-00
Pages
5532-5
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC361269
Subset
IM
Grants
NIAID NIH HHS · AI27307 · United States
NCI NIH HHS · CA42567 · United States
NCI NIH HHS · F32 CA08648 · United States
External Links
PubMed source DOI: 10.1128/mcb.10.10.5532-5535.1990 PMC Full Text View journal details
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