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PMID: 7882984 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The requirement for the basal transcription factor IIE is determined by the helical stability of promoter DNA.

The EMBO journal ·Vol. 14 ·No. 4 ·1995-02-15 ·Pages 810-9

Holstege FC, Tantin D, Carey M, van der Vliet PC, Timmers HT

Abstract

The role of the basal transcription factor TFIIE was investigated in RNA polymerase II transcription reactions reconstituted with purified proteins. Using negatively supercoiled templates, which circumvent the requirement for TFIIH, we observed that transcription from the adenovirus major-late (ML) core promoter is more dependent on TFIIE than transcription from the adenovirus E4 (E4) or mouse mammary tumor virus (MMTV) promoters. For all three promoters, an increase in the ionic strength of the reaction mixtures led to an increased dependence on TFIIE. Analysis of hybrid ML/MMTV promoters showed that the region encompassing the start site, from -10 to +10, dictates this dependence. Transcription from a relaxed E4 template with a pre-melted -8 to +2 region was completely independent of both TFIIE and TFIIH. We propose that on negatively supercoiled templates TFIIE can facilitate promoter melting.

MeSH Terms
Adenoviruses, Human/genetics Base Sequence DNA/chemistry In Vitro Techniques Mammary Tumor Virus, Mouse/genetics Molecular Sequence Data Nucleic Acid Conformation Osmolar Concentration Promoter Regions, Genetic Repetitive Sequences, Nucleic Acid Transcription Factor TFIIH Transcription Factors/metabolism Transcription Factors, TFII Transcription, Genetic
Chemicals
Transcription Factors Transcription Factors, TFII transcription factor TFIIE Transcription Factor TFIIH DNA
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Holstege F C
Laboratory for Physiological Chemistry, Utrecht University, The Netherlands.
Tantin D
Carey M
van der Vliet P C
Timmers H T
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1995-02-15
Pages
810-9
Language
English
Region
England
NLM ID
8208664
PMCID
PMC398147
Subset
IM
Grants
NIGMS NIH HHS · GM07185 · United States
NIGMS NIH HHS · GM46424 · United States
External Links
PubMed source PMC Full Text View journal details
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