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PMID: 1386928 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A carboxyl-terminal-domain kinase associated with RNA polymerase II transcription factor delta from rat liver.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 89 ·No. 16 ·1992-08-15 ·Pages 7476-80

Serizawa H, Conaway RC, Conaway JW

Abstract

We previously purified RNA polymerase II transcription factor delta from rat liver and found that it has an associated DNA-dependent ATPase (dATPase) activity. In this report, we show that delta is also closely associated with a protein kinase activity that catalyzes phosphorylation of the largest subunit of RNA polymerase II. Kinase activity copurifies with transcription and DNA-dependent ATPase (dATPase) activities when delta is analyzed by anion- and cation-exchange HPLC as well as by sucrose gradient sedimentation, arguing that delta possesses all three activities. Phosphorylation of the largest subunits of both rat and yeast RNA polymerase II is stimulated by DNA, whereas phosphorylation of a synthetic peptide containing multiple copies of the carboxyl-terminal heptapeptide repeat is not. Although both ATP and GTP appear to function as phosphate donors, GTP is utilized less than 10% as well as ATP. These findings suggest that delta may exert its action in transcription at least in part through a mechanism involving phosphorylation of the largest subunit of RNA polymerase II.

MeSH Terms
Adenosine Triphosphatases/isolation & purification,metabolism Animals Chromatography, High Pressure Liquid Chromatography, Ion Exchange DNA Helicases DNA-Binding Proteins Electrophoresis, Polyacrylamide Gel Erythroid-Specific DNA-Binding Factors Kinetics Liver/metabolism Macromolecular Substances Molecular Weight Phosphorylation Protein Kinases/isolation & purification,metabolism RNA Polymerase II/isolation & purification,metabolism Rats Transcription Factors/isolation & purification,metabolism
Chemicals
DNA-Binding Proteins Erythroid-Specific DNA-Binding Factors Macromolecular Substances Transcription Factors Protein Kinases RNA Polymerase II Adenosine Triphosphatases DNA Helicases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Serizawa H
Program in Molecular and Cell Biology, Oklahoma Medical Research Foundation, Oklahoma City 73104.
Conaway R C
Conaway J W
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47 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.89.16.7476
Published
1992-08-15
Pages
7476-80
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC49733
Subset
IM
Grants
NIGMS NIH HHS · GM41628 · United States
External Links
PubMed source DOI: 10.1073/pnas.89.16.7476 PMC Full Text View journal details
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