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PMID: 8313889 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A TATA sequence-dependent transcriptional repressor activity associated with mammalian transcription factor IIA.

The EMBO journal ·Vol. 13 ·No. 2 ·1994-01-15 ·Pages 435-45

Aso T, Serizawa H, Conaway RC, Conaway JW

Abstract

In the process of characterizing cellular proteins that modulate basal transcription by RNA polymerase II, we identified a novel repressor activity specific for promoters containing consensus TATA boxes. This activity strongly represses TATA-binding protein (TBP)-dependent transcription initiation from core promoter elements containing a consensus TATA sequence, but activates TBP-dependent transcription from core promoter elements lacking a consensus TATA sequence. Purification of this activity to near homogeneity from rat liver nuclear extracts led to the surprising discovery that it co-purifies closely with mammalian transcription factor IIA (TFIIA). The close association of TATA sequence-dependent transcriptional repressor activity with TFIIA adds a new and unexpected dimension to the already complex picture of this factor's function in transcription by RNA polymerase II.

MeSH Terms
Animals Base Sequence Consensus Sequence DNA Liver/metabolism Male Molecular Sequence Data Promoter Regions, Genetic RNA Polymerase II/metabolism Rats Rats, Sprague-Dawley Repressor Proteins/metabolism TATA Box Transcription Factor TFIIA Transcription Factors/metabolism
Chemicals
Repressor Proteins Transcription Factor TFIIA Transcription Factors DNA RNA Polymerase II
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Aso T
Program in Molecular and Cell Biology, Oklahoma Medical Research Foundation, Oklahoma City 73104.
Serizawa H
Conaway R C
Conaway J W
References (77)
77 references, click to expand
  1. Class II (B) general transcription factor (TFIIB) that binds to the template-committed preinitiation complex is different from general transcription factor BTF3.
    Proc Natl Acad Sci U S A. 1992 Jan 1;89(1):397-401 PMID: 1729710
  2. Diverse transcriptional functions of the multisubunit eukaryotic TFIID complex.
    J Biol Chem. 1992 Jan 15;267(2):679-82 PMID: 1730659
  3. Characterization of cDNA for the large subunit of the transcription initiation factor TFIIF.
    Nature. 1992 Jan 30;355(6359):461-4 PMID: 1734283
  4. A cDNA encoding RAP74, a general initiation factor for transcription by RNA polymerase II.
    Nature. 1992 Jan 30;355(6359):464-7 PMID: 1734284
  5. Isolation of two genes that encode subunits of the yeast transcription factor IIA.
    Science. 1992 Feb 28;255(5048):1127-9 PMID: 1546313
  6. Transcription factor IID mutants defective for interaction with transcription factor IIA.
    Science. 1992 Feb 28;255(5048):1130-2 PMID: 1546314
  7. Mechanism of assembly of the RNA polymerase II preinitiation complex. Transcription factors delta and epsilon promote stable binding of the transcription apparatus to the initiator element.
    J Biol Chem. 1992 May 15;267(14):10142-8 PMID: 1577784
  8. The acidic transcriptional activator GAL-VP16 acts on preformed template-committed complexes.
    EMBO J. 1992 Jun;11(6):2229-40 PMID: 1376247
  9. Identification of a coactivator that increases activation of transcription by serum response factor and GAL4-VP16 in vitro.
    Proc Natl Acad Sci U S A. 1992 Jun 15;89(12):5291-5 PMID: 1608937
  10. Functional binding of the "TATA" box binding component of transcription factor TFIID to the -30 region of TATA-less promoters.
    Proc Natl Acad Sci U S A. 1992 Jul 1;89(13):5814-8 PMID: 1321424
  11. Identification of three mammalian proteins that bind to the yeast TATA box protein TFIID.
    Gene Expr. 1992;2(2):99-110 PMID: 1633441
  12. Dr1, a TATA-binding protein-associated phosphoprotein and inhibitor of class II gene transcription.
    Cell. 1992 Aug 7;70(3):477-89 PMID: 1339312
  13. TFIIA is required for in vitro transcription of mammalian U6 genes by RNA polymerase III.
    J Biol Chem. 1992 Aug 15;267(23):16359-64 PMID: 1644819
  14. cDNA clone encoding Drosophila transcription factor TFIID.
    Proc Natl Acad Sci U S A. 1990 Dec;87(23):9148-52 PMID: 2123550
  15. Recombinant yeast TFIID, a general transcription factor, mediates activation by the gene-specific factor USF in a chromatin assembly assay.
    Proc Natl Acad Sci U S A. 1990 Dec;87(23):9153-7 PMID: 2251256
  16. A mediator required for activation of RNA polymerase II transcription in vitro.
    Nature. 1991 Apr 4;350(6317):436-8 PMID: 2011193
  17. Transcription initiated by RNA polymerase II and transcription factors from liver. Structure and action of transcription factors epsilon and tau.
    J Biol Chem. 1991 Apr 25;266(12):7804-11 PMID: 2019603
  18. Affinity purification of transcription factor IIA from HeLa cell nuclear extracts.
    EMBO J. 1991 Aug;10(8):2305-10 PMID: 2065666
  19. Isolation of coactivators associated with the TATA-binding protein that mediate transcriptional activation.
    Cell. 1991 Aug 9;66(3):563-76 PMID: 1907890
  20. Evidence for a factor required for transcriptional stimulation by the chimeric acidic activator GAL-VP16 in HeLa cell extracts.
    Proc Natl Acad Sci U S A. 1991 Sep 1;88(17):7674-8 PMID: 1881909
  21. Activation of class II gene transcription by regulatory factors is potentiated by a novel activity.
    Cell. 1991 Sep 6;66(5):981-93 PMID: 1889091
  22. The yeast general transcription factor TFIIA is composed of two polypeptide subunits.
    J Biol Chem. 1991 Oct 15;266(29):19320-7 PMID: 1918049
  23. Family of proteins that interact with TFIID and regulate promoter activity.
    Cell. 1991 Nov 1;67(3):557-67 PMID: 1934060
  24. Multiple factors required for accurate initiation of transcription by purified RNA polymerase II.
    J Biol Chem. 1980 Dec 25;255(24):11992-6 PMID: 7440580
  25. Separation and characterization of factors mediating accurate transcription by RNA polymerase II.
    J Biol Chem. 1982 Dec 10;257(23):14419-27 PMID: 7142220
  26. Formation of stable preinitiation complexes between eukaryotic class B transcription factors and promoter sequences.
    Nature. 1983 Feb 24;301(5902):680-6 PMID: 6828151
  27. Is actin a transcription initiation factor for RNA polymerase B?
    EMBO J. 1984 Oct;3(10):2363-71 PMID: 6499833
  28. Interaction of a gene-specific transcription factor with the adenovirus major late promoter upstream of the TATA box region.
    Cell. 1985 Nov;43(1):165-75 PMID: 4075392
  29. An RNA polymerase II transcription factor binds to an upstream element in the adenovirus major late promoter.
    Cell. 1985 Dec;43(2 Pt 1):439-48 PMID: 4075400
  30. Purification and characterization of a specific RNA polymerase II transcription factor.
    J Biol Chem. 1986 Feb 15;261(5):2003-13 PMID: 3944124
  31. Specific interaction between a transcription factor and the upstream element of the adenovirus-2 major late promoter.
    EMBO J. 1985 Dec 16;4(13A):3563-70 PMID: 4092688
  32. Purification of a factor specific for the upstream element of the adenovirus-2 major late promoter.
    EMBO J. 1986 Oct;5(10):2577-84 PMID: 3023048
  33. Nucleosomes inhibit the initiation of transcription but allow chain elongation with the displacement of histones.
    Cell. 1987 Apr 24;49(2):203-10 PMID: 3568125
  34. An RNA polymerase II transcription system from rat liver. Purification of an essential component.
    J Biol Chem. 1987 Jun 15;262(17):8293-7 PMID: 3597375
  35. Function of a yeast TATA element-binding protein in a mammalian transcription system.
    Nature. 1988 Jul 7;334(6177):37-42 PMID: 3290687
  36. A multisubunit transcription factor essential for accurate initiation by RNA polymerase II.
    J Biol Chem. 1989 Feb 5;264(4):2357-62 PMID: 2914911
  37. Five intermediate complexes in transcription initiation by RNA polymerase II.
    Cell. 1989 Feb 24;56(4):549-61 PMID: 2917366
  38. Purification of a yeast TATA box-binding protein that exhibits human transcription factor IID activity.
    Proc Natl Acad Sci U S A. 1989 Jul;86(13):4843-7 PMID: 2662184
  39. Transcriptional regulation in mammalian cells by sequence-specific DNA binding proteins.
    Science. 1989 Jul 28;245(4916):371-8 PMID: 2667136
  40. Yeast TATA-binding protein TFIID binds to TATA elements with both consensus and nonconsensus DNA sequences.
    Proc Natl Acad Sci U S A. 1989 Aug;86(15):5718-22 PMID: 2569738
  41. Molecular mechanisms of transcriptional regulation in yeast.
    Annu Rev Biochem. 1989;58:1051-77 PMID: 2673007
  42. Eukaryotic transcriptional regulatory proteins.
    Annu Rev Biochem. 1989;58:799-839 PMID: 2673023
  43. Isolation of the gene encoding the yeast TATA binding protein TFIID: a gene identical to the SPT15 suppressor of Ty element insertions.
    Cell. 1989 Sep 22;58(6):1173-81 PMID: 2550146
  44. Cloning and structure of a yeast gene encoding a general transcription initiation factor TFIID that binds to the TATA box.
    Nature. 1989 Sep 28;341(6240):299-303 PMID: 2677740
  45. Identification of a yeast protein homologous in function to the mammalian general transcription factor, TFIIA.
    EMBO J. 1989 Nov;8(11):3379-82 PMID: 2684641
  46. Cloning of the gene encoding the yeast protein BTF1Y, which can substitute for the human TATA box-binding factor.
    Proc Natl Acad Sci U S A. 1989 Dec;86(24):9803-7 PMID: 2690073
  47. Transcription initiated by RNA polymerase II and purified transcription factors from liver. Cooperative action of transcription factors tau and epsilon in initial complex formation.
    J Biol Chem. 1990 May 5;265(13):7552-8 PMID: 2332442
  48. Transcription initiated by RNA polymerase II and purified transcription factors from liver. Transcription factors alpha, beta gamma, and delta promote formation of intermediates in assembly of the functional preinitiation complex.
    J Biol Chem. 1990 May 5;265(13):7559-63 PMID: 1692022
  49. Transcription initiated by RNA polymerase II and purified transcription factors from liver. A complex set of promoter sequences governs formation of the initial complex.
    J Biol Chem. 1990 May 5;265(13):7564-9 PMID: 2159003
  50. Cloning of a transcriptionally active human TATA binding factor.
    Science. 1990 Jun 29;248(4963):1646-50 PMID: 2194289
  51. Isolation and characterization of the Drosophila gene encoding the TATA box binding protein, TFIID.
    Cell. 1990 Jun 29;61(7):1179-86 PMID: 2194666
  52. Mechanism of transcriptional activation by Sp1: evidence for coactivators.
    Cell. 1990 Jun 29;61(7):1187-97 PMID: 2194667
  53. Selective inhibition of activated but not basal transcription by the acidic activation domain of VP16: evidence for transcriptional adaptors.
    Cell. 1990 Jun 29;61(7):1199-208 PMID: 2163758
  54. A novel mediator between activator proteins and the RNA polymerase II transcription apparatus.
    Cell. 1990 Jun 29;61(7):1209-15 PMID: 2163759
  55. Yeast and human TATA-binding proteins have nearly identical DNA sequence requirements for transcription in vitro.
    Mol Cell Biol. 1990 Aug;10(8):3859-67 PMID: 2196437
  56. RNA polymerase B (II) and general transcription factors.
    Annu Rev Biochem. 1990;59:711-54 PMID: 2197989
  57. A carboxyl-terminal-domain kinase associated with RNA polymerase II transcription factor delta from rat liver.
    Proc Natl Acad Sci U S A. 1992 Aug 15;89(16):7476-80 PMID: 1386928
  58. Holo-TFIID supports transcriptional stimulation by diverse activators and from a TATA-less promoter.
    Genes Dev. 1992 Oct;6(10):1964-74 PMID: 1398073
  59. TFIIA induces conformational changes in TFIID via interactions with the basic repeat.
    Mol Cell Biol. 1992 Nov;12(11):5189-96 PMID: 1406690
  60. Reconstitution of transcription with five purified initiation factors and RNA polymerase II from Saccharomyces cerevisiae.
    J Biol Chem. 1992 Nov 15;267(32):23376-82 PMID: 1331084
  61. The carboxyl terminus of RAP30 is similar in sequence to region 4 of bacterial sigma factors and is required for function.
    J Biol Chem. 1992 Nov 25;267(33):23942-9 PMID: 1429731
  62. Initiation of transcription by RNA polymerase II: a multi-step process.
    Prog Nucleic Acid Res Mol Biol. 1993;44:67-108 PMID: 8434126
  63. Factors (TAFs) required for activated transcription interact with TATA box-binding protein conserved core domain.
    Genes Dev. 1993 Feb;7(2):180-7 PMID: 8436290
  64. Structure(?) and function of acidic transcription activators.
    Cell. 1993 Feb 26;72(4):481-3 PMID: 8440015
  65. Distinct TFIID complexes mediate the effect of different transcriptional activators.
    EMBO J. 1993 Feb;12(2):489-99 PMID: 8440239
  66. Largest subunit of Drosophila transcription factor IID directs assembly of a complex containing TBP and a coactivator.
    Nature. 1993 Apr 8;362(6420):511-7 PMID: 8464492
  67. Drosophila 230-kD TFIID subunit, a functional homolog of the human cell cycle gene product, negatively regulates DNA binding of the TATA box-binding subunit of TFIID.
    Genes Dev. 1993 Jun;7(6):1033-46 PMID: 8504928
  68. General initiation factors for RNA polymerase II.
    Annu Rev Biochem. 1993;62:161-90 PMID: 8352588
  69. An alternative pathway for transcription initiation involving TFII-I.
    Nature. 1993 Sep 23;365(6444):355-9 PMID: 8377828
  70. A downstream initiation element required for efficient TATA box binding and in vitro function of TFIID.
    Nature. 1990 Nov 1;348(6296):86-8 PMID: 2234067
  71. Factors involved in specific transcription by mammalian RNA polymerase II: role of transcription factors IIA, IID, and IIB during formation of a transcription-competent complex.
    Mol Cell Biol. 1990 Dec;10(12):6335-47 PMID: 2247058
  72. Transcription from a TATA-less promoter requires a multisubunit TFIID complex.
    Genes Dev. 1991 Nov;5(11):1935-45 PMID: 1657708
  73. Structure and functional properties of human general transcription factor IIE.
    Nature. 1991 Dec 5;354(6352):369-73 PMID: 1956398
  74. Structural motifs and potential sigma homologies in the large subunit of human general transcription factor TFIIE.
    Nature. 1991 Dec 5;354(6352):398-401 PMID: 1956403
  75. Conserved sequence motifs in the small subunit of human general transcription factor TFIIE.
    Nature. 1991 Dec 5;354(6352):401-4 PMID: 1956404
  76. Coactivators for a proline-rich activator purified from the multisubunit human TFIID complex.
    Genes Dev. 1991 Dec;5(12A):2212-24 PMID: 1748279
  77. Factors involved in specific transcription by mammalian RNA polymerase II: purification and analysis of transcription factor IIA and identification of transcription factor IIJ.
    Mol Cell Biol. 1992 Jan;12(1):413-21 PMID: 1729613
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1994-01-15
Pages
435-45
Language
English
Region
England
NLM ID
8208664
PMCID
PMC394826
Subset
IM
Grants
NIGMS NIH HHS · GM41628 · United States
External Links
PubMed source PMC Full Text View journal details
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