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PMID: 1406690 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

TFIIA induces conformational changes in TFIID via interactions with the basic repeat.

Molecular and cellular biology ·Vol. 12 ·No. 11 ·1992-11-00 ·Pages 5189-96

Lee DK, DeJong J, Hashimoto S, Horikoshi M, Roeder RG

Abstract

DNA-binding studies with Saccharomyces cerevisiae TFIID point mutants indicated that TFIIA interacts with the basic repeat region of TFIID and induces structural changes. The latter was shown by the ability of TFIIA to compensate for TFIID point mutants defective for DNA binding. Interaction with TFIIA also rendered TFIID binding temperature independent, thus mimicking the effect of removing the nonconserved N terminus of TFIID. In addition, N-terminal truncation of the TFIID point mutants defective for DNA binding mimicked the ability of TFIIA to restore DNA binding of those mutants. Taken together, these results suggest that TFIIA enhances TFIID binding to DNA by eliminating an otherwise inhibitory effect of the nonconserved N terminus of TFIID. Furthermore, analyses of TFIID contact points on DNA and binding studies with TATA-containing oligonucleotide probes showed that TFIIA decreases the effect of sequences flanking the adenovirus major late TATA element on TFIID binding to DNA, suggesting a possible role of TFIIA in allowing TFIID to recognize a wider variety of promoters.

MeSH Terms
Base Sequence DNA/metabolism Humans Molecular Sequence Data Mutation Promoter Regions, Genetic Protein Binding Protein Conformation Repetitive Sequences, Nucleic Acid TATA Box Temperature Transcription Factor TFIIA Transcription Factor TFIID Transcription Factors/chemistry,metabolism Transcription, Genetic
Chemicals
Transcription Factor TFIIA Transcription Factor TFIID Transcription Factors DNA
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Lee D K
Laboratory of Biochemistry and Molecular Biology, Rockefeller University, New York, New York 10021.
DeJong J
Hashimoto S
Horikoshi M
Roeder R G
References (54)
54 references, click to expand
  1. Sp1 activates transcription without enhancing DNA-binding activity of the TATA box factor.
    Mol Cell Biol. 1989 Aug;9(8):3299-307 PMID: 2677669
  2. Dominant negative mutations in yeast TFIID define a bipartite DNA-binding region.
    Cell. 1991 Apr 19;65(2):349-57 PMID: 2015629
  3. Five intermediate complexes in transcription initiation by RNA polymerase II.
    Cell. 1989 Feb 24;56(4):549-61 PMID: 2917366
  4. Physical analysis of transcription preinitiation complex assembly on a class II gene promoter.
    Science. 1988 Sep 9;241(4871):1335-8 PMID: 3413495
  5. Factors involved in specific transcription in mammalian RNA polymerase II. Functional analysis of initiation factors IIA and IID and identification of a new factor operating at sequences downstream of the initiation site.
    J Biol Chem. 1987 Mar 5;262(7):3322-30 PMID: 3818643
  6. Interaction of a gene-specific transcription factor with the adenovirus major late promoter upstream of the TATA box region.
    Cell. 1985 Nov;43(1):165-75 PMID: 4075392
  7. Isolation of two genes that encode subunits of the yeast transcription factor IIA.
    Science. 1992 Feb 28;255(5048):1127-9 PMID: 1546313
  8. Factors involved in specific transcription by mammalian RNA polymerase II. Identification and characterization of factor IIH.
    J Biol Chem. 1992 Feb 5;267(4):2786-93 PMID: 1733973
  9. TFIID binds in the minor groove of the TATA box.
    Cell. 1991 Dec 20;67(6):1231-40 PMID: 1760847
  10. Direct interaction between adenovirus E1A protein and the TATA box binding transcription factor IID.
    Proc Natl Acad Sci U S A. 1991 Jun 15;88(12):5124-8 PMID: 1828892
  11. Striking homology of the 'variable' N-terminal as well as the 'conserved core' domains of the mouse and human TATA-factors (TFIID).
    Nucleic Acids Res. 1991 Jul 25;19(14):3861-5 PMID: 1861978
  12. The conserved carboxy-terminal domain of Saccharomyces cerevisiae TFIID is sufficient to support normal cell growth.
    Mol Cell Biol. 1991 Oct;11(10):4809-21 PMID: 1922021
  13. Purification and interaction properties of the human RNA polymerase B(II) general transcription factor BTF2.
    J Biol Chem. 1991 Nov 5;266(31):20940-5 PMID: 1939143
  14. A highly conserved domain of TFIID displays species specificity in vivo.
    Cell. 1991 Apr 19;65(2):333-40 PMID: 2015627
  15. Requirement for acidic amino acid residues immediately N-terminal to the conserved domain of Saccharomyces cerevisiae TFIID.
    EMBO J. 1991 Jul;10(7):1843-52 PMID: 2050121
  16. Activators and targets.
    Nature. 1990 Jul 26;346(6282):329-31 PMID: 2142753
  17. Direct and selective binding of an acidic transcriptional activation domain to the TATA-box factor TFIID.
    Nature. 1990 Jun 28;345(6278):783-6 PMID: 2193231
  18. Cloning of a transcriptionally active human TATA binding factor.
    Science. 1990 Jun 29;248(4963):1646-50 PMID: 2194289
  19. Isolation and characterization of the Drosophila gene encoding the TATA box binding protein, TFIID.
    Cell. 1990 Jun 29;61(7):1179-86 PMID: 2194666
  20. RNA polymerase B (II) and general transcription factors.
    Annu Rev Biochem. 1990;59:711-54 PMID: 2197989
  21. cDNA clone encoding Drosophila transcription factor TFIID.
    Proc Natl Acad Sci U S A. 1990 Dec;87(23):9148-52 PMID: 2123550
  22. Analysis of structure-function relationships of yeast TATA box binding factor TFIID.
    Cell. 1990 Jun 29;61(7):1171-8 PMID: 2194665
  23. Arabidopsis thaliana contains two genes for TFIID.
    Nature. 1990 Jul 26;346(6282):390-4 PMID: 2197561
  24. Factors involved in specific transcription by mammalian RNA polymerase II: role of transcription factors IIA, IID, and IIB during formation of a transcription-competent complex.
    Mol Cell Biol. 1990 Dec;10(12):6335-47 PMID: 2247058
  25. Functional domains and upstream activation properties of cloned human TATA binding protein.
    Science. 1990 Jun 29;248(4963):1625-30 PMID: 2363050
  26. Isolation of the gene encoding the yeast TATA binding protein TFIID: a gene identical to the SPT15 suppressor of Ty element insertions.
    Cell. 1989 Sep 22;58(6):1173-81 PMID: 2550146
  27. Purification of a yeast TATA box-binding protein that exhibits human transcription factor IID activity.
    Proc Natl Acad Sci U S A. 1989 Jul;86(13):4843-7 PMID: 2662184
  28. Cloning of the Schizosaccharomyces pombe TFIID gene reveals a strong conservation of functional domains present in Saccharomyces cerevisiae TFIID.
    Genes Dev. 1990 Jul;4(7):1141-8 PMID: 2210373
  29. Factors involved in specific transcription by mammalian RNA polymerase II: identification of general transcription factor TFIIG.
    Proc Natl Acad Sci U S A. 1990 Dec;87(23):9158-62 PMID: 2251257
  30. Highly conserved core domain and unique N terminus with presumptive regulatory motifs in a human TATA factor (TFIID).
    Nature. 1990 Jul 26;346(6282):387-90 PMID: 2374612
  31. Stability of transcription complexes on class II genes.
    Mol Cell Biol. 1989 Jan;9(1):342-4 PMID: 2494433
  32. An RNA polymerase II transcription factor has an associated DNA-dependent ATPase (dATPase) activity strongly stimulated by the TATA region of promoters.
    Proc Natl Acad Sci U S A. 1989 Oct;86(19):7356-60 PMID: 2552440
  33. SPT15, the gene encoding the yeast TATA binding factor TFIID, is required for normal transcription initiation in vivo.
    Cell. 1989 Sep 22;58(6):1183-91 PMID: 2673545
  34. Cloning and structure of a yeast gene encoding a general transcription initiation factor TFIID that binds to the TATA box.
    Nature. 1989 Sep 28;341(6240):299-303 PMID: 2677740
  35. Yeast TATA-box transcription factor gene.
    Proc Natl Acad Sci U S A. 1989 Oct;86(20):7785-9 PMID: 2682626
  36. Cloning of the gene encoding the yeast protein BTF1Y, which can substitute for the human TATA box-binding factor.
    Proc Natl Acad Sci U S A. 1989 Dec;86(24):9803-7 PMID: 2690073
  37. Factors involved in specific transcription by mammalian RNA polymerase II: purification, genetic specificity, and TATA box-promoter interactions of TFIID.
    Mol Cell Biol. 1988 Oct;8(10):4028-40 PMID: 3185540
  38. A yeast activity can substitute for the HeLa cell TATA box factor.
    Nature. 1988 Jul 7;334(6177):77-80 PMID: 3290688
  39. Transcription factor ATF interacts with the TATA factor to facilitate establishment of a preinitiation complex.
    Cell. 1988 Sep 23;54(7):1033-42 PMID: 3416354
  40. Purification and characterization of a specific RNA polymerase II transcription factor.
    J Biol Chem. 1986 Feb 15;261(5):2003-13 PMID: 3944124
  41. Interactions between RNA polymerase II, factors, and template leading to accurate transcription.
    J Biol Chem. 1984 Feb 25;259(4):2509-16 PMID: 6698978
  42. A bipartite DNA binding domain composed of direct repeats in the TATA box binding factor TFIID.
    Proc Natl Acad Sci U S A. 1992 Apr 1;89(7):2844-8 PMID: 1557391
  43. Factors involved in specific transcription by mammalian RNA polymerase II: purification and analysis of transcription factor IIA and identification of transcription factor IIJ.
    Mol Cell Biol. 1992 Jan;12(1):413-21 PMID: 1729613
  44. Transcription factor TFIID induces DNA bending upon binding to the TATA element.
    Proc Natl Acad Sci U S A. 1992 Feb 1;89(3):1060-4 PMID: 1736286
  45. Interaction of TFIID in the minor groove of the TATA element.
    Cell. 1991 Dec 20;67(6):1241-50 PMID: 1760848
  46. The complexities of eukaryotic transcription initiation: regulation of preinitiation complex assembly.
    Trends Biochem Sci. 1991 Nov;16(11):402-8 PMID: 1776168
  47. Adenovirus E1A activation domain binds the basic repeat in the TATA box transcription factor.
    Cell. 1991 Oct 18;67(2):365-76 PMID: 1833071
  48. Activation of class II gene transcription by regulatory factors is potentiated by a novel activity.
    Cell. 1991 Sep 6;66(5):981-93 PMID: 1889091
  49. The yeast general transcription factor TFIIA is composed of two polypeptide subunits.
    J Biol Chem. 1991 Oct 15;266(29):19320-7 PMID: 1918049
  50. Family of proteins that interact with TFIID and regulate promoter activity.
    Cell. 1991 Nov 1;67(3):557-67 PMID: 1934060
  51. Affinity purification of transcription factor IIA from HeLa cell nuclear extracts.
    EMBO J. 1991 Aug;10(8):2305-10 PMID: 2065666
  52. Two distinct domains in the yeast transcription factor IID and evidence for a TATA box-induced conformational change.
    Mol Cell Biol. 1991 Jan;11(1):63-74 PMID: 1986253
  53. Functional differences between yeast and human TFIID are localized to the highly conserved region.
    Cell. 1991 Apr 19;65(2):341-8 PMID: 2015628
  54. Identification of a yeast protein homologous in function to the mammalian general transcription factor, TFIIA.
    EMBO J. 1989 Nov;8(11):3379-82 PMID: 2684641
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.12.11.5189-5196.1992
Published
1992-11-00
Pages
5189-96
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC360452
Subset
IM
Grants
NIAID NIH HHS · AI27397 · United States
NCI NIH HHS · CA42567 · United States
NIGMS NIH HHS · GM45258 · United States
External Links
PubMed source DOI: 10.1128/mcb.12.11.5189-5196.1992 PMC Full Text View journal details
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