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PMID: 8524642 Published · ppublish English Journal Article

The amino-terminal tails of the core histones and the translational position of the TATA box determine TBP/TFIIA association with nucleosomal DNA.

Nucleic acids research ·Vol. 23 ·No. 22 ·1995-11-25 ·Pages 4557-64

Godde JS, Nakatani Y, Wolffe AP

Abstract

We establish that the TATA binding protein (TBP) in the presence of TFIIA recognizes the TATA box in nucleosomal DNA dependent on the dissociation of the amino-terminal tails of the core histones from the nucleosome and the position of the TATA box within the nucleosome. We examine TBP/TFIIA access to the TATA box with this sequence placed in four distinct rotational frames with reference to the histone surface and at three distinct translational positions at the edge, side and dyad axis of the nucleosome. Under our experimental conditions, we find that the preferential translational position at which TBP/TFIIA can bind the TATA box is within linker DNA at the edge of the nucleosome and that binding is facilitated if contacts made by the amino-terminal tails of the histones with nucleosomal DNA are eliminated. TBP/TFIIA binding to DNA at the edge of the nucleosome occurs with the TATA box in all four rotational positions. This is indicative of TBP/TFIIA association directing the dissociation of the TATA box from the surface of the histone octamer.

MeSH Terms
Animals Base Sequence Binding Sites DNA, Ribosomal/chemistry,genetics,metabolism DNA-Binding Proteins/metabolism Histones/chemistry,metabolism Models, Structural Molecular Sequence Data Nucleic Acid Conformation Nucleosomes/metabolism Protein Biosynthesis Protein Conformation RNA, Ribosomal, 5S/genetics TATA Box TATA-Box Binding Protein Transcription Factor TFIIA Transcription Factors/metabolism Xenopus
Chemicals
DNA, Ribosomal DNA-Binding Proteins Histones Nucleosomes RNA, Ribosomal, 5S TATA-Box Binding Protein Transcription Factor TFIIA Transcription Factors
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Godde J S
Laboratory of Molecular Embryology, National Institute of Child Health and Human Development, NIH, Bethesda, MD 20892-2710, USA.
Nakatani Y
Wolffe A P
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/23.22.4557
Published
1995-11-25
Pages
4557-64
Language
English
Region
England
NLM ID
0411011
PMCID
PMC307425
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/23.22.4557 PMC Full Text View journal details
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