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PMID: 8052665 Published · ppublish English Journal Article

Contacts of the globular domain of histone H5 and core histones with DNA in a "chromatosome".

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 91 ·No. 16 ·1994-08-02 ·Pages 7817-21

Hayes JJ, Pruss D, Wolffe AP

Abstract

The globular domain of histone H5 is found to asymmetrically associate with a nucleosome core including the Xenopus borealis somatic 5S RNA gene. Histones H2A and H2B are required for association of histone H5. Strong crosslinking of the globular domain of histone H5 to the 5S DNA in the nucleosome occurs at a single site to one side of the dyad axis. This site is also in contact with the core histones, and the interactions of the core histones with 5S DNA change as a result of association of the globular domain of histone H5. We discuss evidence for an allosteric change in core histone-5S DNA interactions following the association of the linker histone in the nucleosome.

MeSH Terms
Animals Cross-Linking Reagents DNA, Ribosomal/chemistry Histones/chemistry Models, Structural Molecular Conformation Nucleosomes/chemistry RNA, Ribosomal, 5S/genetics Xenopus
Chemicals
Cross-Linking Reagents DNA, Ribosomal Histones Nucleosomes RNA, Ribosomal, 5S
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Hayes J J
Laboratory of Molecular Embryology, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892.
Pruss D
Wolffe A P
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.91.16.7817
Published
1994-08-02
Pages
7817-21
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC44493
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.91.16.7817 PMC Full Text View journal details
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