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PMID: 8522580 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Interaction between BiP and Sec63p is required for the completion of protein translocation into the ER of Saccharomyces cerevisiae.

The Journal of cell biology ·Vol. 131 ·No. 5 ·1995-12-00 ·Pages 1163-71

Lyman SK, Schekman R

Abstract

To clarify the roles of Kar2p (BiP) and Sec63p in translocation across the ER membrane in Saccharomyces cerevisiae, we have utilized mutant alleles of the essential genes that encode these proteins: kar2-203 and sec63-1. Sanders et al. (Sanders, S. L., K. M. Whitfield, J. P. Vogel, M. D. Rose, and R. W. Schekman. 1992. Cell. 69:353-365) showed that the translocation defect of the kar2-203 mutant lies in the inability of the precursor protein to complete its transit across the membrane, suggesting that the lumenal hsp70 homologue Kar2p (BiP) binds the transiting polypeptide in order to facilitate its passage through the pore. We now show that mutation of a conserved residue (A181-->T) (Nelson, M. K., T. Kurihara, and P. Silver. 1993. Genetics. 134:159-173) in the lumenal DnaJ box of Sec63p (sec63-1) results in an in vitro phenotype that mimics the precursor stalling defect of kar2-203. We demonstrate by several criteria that this phenotype results specifically from a defect in the lumenal interaction between Sec63p and BiP: Neither a sec62-1 mutant nor a mutation in the cytosolically exposed domain of Sec63p causes precursor stalling, and interaction of the sec63-1 mutant with the membranebound components of the translocation apparatus is unimpaired. Additionally, dominant KAR2 suppressors of sec63-1 partially relieve the stalling defect. Thus, proper interaction between BiP and Sec63p is necessary to allow the precursor polypeptide to complete its transit across the membrane.

MeSH Terms
Binding Sites Endoplasmic Reticulum/metabolism Fungal Proteins/metabolism HSP70 Heat-Shock Proteins/metabolism Heat-Shock Proteins Membrane Proteins/metabolism Membrane Transport Proteins Mutation Protein Precursors/metabolism Protein Sorting Signals/metabolism Saccharomyces cerevisiae/genetics,metabolism Saccharomyces cerevisiae Proteins
Chemicals
Fungal Proteins HSP70 Heat-Shock Proteins Heat-Shock Proteins KAR2 protein, yeast Membrane Proteins Membrane Transport Proteins Protein Precursors Protein Sorting Signals SEC63 protein, S cerevisiae Saccharomyces cerevisiae Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Lyman S K
Department of Molecular and Cell Biology, Howard Hughes Medical Institute, University of California, Berkeley 94720, USA.
Schekman R
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.131.5.1163
Published
1995-12-00
Pages
1163-71
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2120636
Subset
IM
Grants
NIGMS NIH HHS · GM 26755 · United States
External Links
PubMed source DOI: 10.1083/jcb.131.5.1163 PMC Full Text View journal details
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