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PMID: 8514125 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Extragenic suppressors of mutations in the cytoplasmic C terminus of SEC63 define five genes in Saccharomyces cerevisiae.

Genetics ·Vol. 134 ·No. 1 ·1993-05-00 ·Pages 159-73

Nelson MK, Kurihara T, Silver PA

Abstract

Mutations in the SEC63 gene of Saccharomyces cerevisiae affect both nuclear protein localization and translocation of proteins into the endoplasmic reticulum. We now report the isolation of suppressors of sec63-101 (formerly npl1-1), a temperature-sensitive allele of SEC63. Five complementation groups of extragenic mutations, son1-son5 (suppressor of npl1-1), were identified among the recessive suppressors. The son mutations are specific to SEC63, are not bypass suppressors, and are not new alleles of previously identified secretory (SEC61, SEC62, KAR2) or nuclear protein localization genes (NPL3, NPL4, NPL6). son1 mutations show regional specificity of suppression of sec63 alleles. At low temperatures, son1 mutants grow slowly and show partial mislocalization of nuclear antigens. The SON1 gene maps to chromosome IV and encodes a nuclear protein of 531 amino acids that contains two acidic stretches and a putative nuclear localization sequence. We show that son1 mutations suppress sec63-101 by elimination of Son1p function.

Related Genes
SEC63 SON1
MeSH Terms
Alleles Amino Acid Sequence Base Sequence Chromosome Mapping Cloning, Molecular DNA, Fungal/genetics Fungal Proteins/genetics,metabolism Genes, Fungal Glycerol/metabolism Molecular Sequence Data Mutation Nuclear Proteins/genetics,metabolism Saccharomyces cerevisiae/genetics,growth & development,metabolism Suppression, Genetic
Chemicals
DNA, Fungal Fungal Proteins Nuclear Proteins Glycerol
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Nelson M K
Department of Molecular Biology, Princeton University, New Jersey 08544-1014.
Kurihara T
Silver P A
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47 references, click to expand
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/134.1.159
Published
1993-05-00
Pages
159-73
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1205419
Subset
IM
Grants
NIGMS NIH HHS · GM07312 · United States
NIGMS NIH HHS · GM07388-13 · United States
Databases
GENBANK
L00928, L07815, L07816, L07817, L07818, L07819, L07820, X69829, X69830, X69831
External Links
PubMed source DOI: 10.1093/genetics/134.1.159 PMC Full Text View journal details
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