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PMID: 2592400 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Identification of a human protein that interacts with nuclear localization signals.

The Journal of cell biology ·Vol. 109 ·No. 6 Pt 1 ·1989-12-00 ·Pages 2623-32

Li RH, Thomas JO

Abstract

Through a series of label transfer experiments, we have identified a HeLa cell nuclear protein that interacts with nuclear localization signals (NLSs). The protein has a molecular weight of 66,000 and an isoelectric point of approximately 6. It associates with a synthetic peptide that contains the SV-40 T antigen NLS peptide but not with an analogous peptide in which an asparagine is substituted for an essential lysine (un-NLS peptide). In addition to these peptides, several proteins have been tested as label donors. With the proteins, there is a correlation between nuclear localization (assayed with lysolecithin-permeabilized cells) and label transfer to the 66-kD protein. The NLS peptide (but not the un-NLS peptide) competes with the proteins in label transfer experiments, but neither wheat germ agglutinin nor ATP has an effect. These results suggest that the 66-kD protein functions as an NLS receptor in the first step of nuclear localization. In the course of this work, we have observed that the Staphylococcus aureus protein A is a strongly karyophilic protein. Its dramatic nuclear localization properties suggest that it may have multiple copies of an NLS.

MeSH Terms
Amino Acid Sequence Base Sequence Cell Membrane Permeability Cell Nucleus/metabolism,ultrastructure Electrophoresis, Gel, Two-Dimensional Electrophoresis, Polyacrylamide Gel HeLa Cells/metabolism Humans Lysophosphatidylcholines Molecular Sequence Data Molecular Weight Nuclear Localization Signals Nuclear Proteins/genetics,isolation & purification,metabolism Oligonucleotide Probes Staphylococcal Protein A/metabolism
Chemicals
Lysophosphatidylcholines Nuclear Localization Signals Nuclear Proteins Oligonucleotide Probes Staphylococcal Protein A
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Li R H
Department of Biochemistry, New York University Medical School 10016.
Thomas J O
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.109.6.2623
Published
1989-12-00
Pages
2623-32
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2115907
Subset
IM
Grants
NIGMS NIH HHS · GM28747 · United States
External Links
PubMed source DOI: 10.1083/jcb.109.6.2623 PMC Full Text View journal details
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