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PMID: 8257795 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Structural and functional characterization of Sec66p, a new subunit of the polypeptide translocation apparatus in the yeast endoplasmic reticulum.

Molecular biology of the cell ·Vol. 4 ·No. 9 ·1993-09-00 ·Pages 931-9

Feldheim D, Yoshimura K, Admon A, Schekman R

Abstract

SEC66 encodes the 31.5-kDa glycoprotein of the Sec63p complex, an integral endoplasmic reticulum membrane protein complex required for translocation of presecretory proteins in Saccharomyces cerevisiae. DNA sequence analysis of SEC66 predicts a 23-kDa protein with no obvious NH2-terminal signal sequence but with one domain of sufficient length and hydrophobicity to span a lipid bilayer. Antibodies directed against a recombinant form of Sec66p were used to confirm the membrane location of Sec66p and that Sec66p is a glycoprotein of 31.5 kDa. A null mutation in SEC66 renders yeast cells temperature sensitive for growth. sec66 cells accumulate some secretory precursors at a permissive temperature and a variety of precursors at the restrictive temperature. sec66 cells show defects in Sec63p complex formation. Because sec66 cells affect the translocation of some, but not all secretory precursor polypeptides, the role of Sec66p may be to interact with the signal peptide of presecretory proteins.

MeSH Terms
Amino Acid Sequence Base Sequence DNA, Fungal/metabolism Endoplasmic Reticulum/metabolism Fungal Proteins/biosynthesis,genetics,metabolism Genes, Fungal Immune Sera Lipid Bilayers Membrane Glycoproteins/biosynthesis,genetics,metabolism Molecular Sequence Data Peptides/chemical synthesis,immunology Saccharomyces cerevisiae/genetics,metabolism Saccharomyces cerevisiae Proteins
Chemicals
DNA, Fungal Fungal Proteins Immune Sera Lipid Bilayers Membrane Glycoproteins Peptides SEC66 protein, S cerevisiae Saccharomyces cerevisiae Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Feldheim D
Department of Molecular and Cell Biology, Howard Hughes Medical Institute, University of California at Berkeley 94720.
Yoshimura K
Admon A
Schekman R
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.4.9.931
Published
1993-09-00
Pages
931-9
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC275723
Subset
IM
Databases
GENBANK
U00797
External Links
PubMed source DOI: 10.1091/mbc.4.9.931 PMC Full Text View journal details
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