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PMID: 8389977 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Growth-related expression of ribosomal protein genes in Saccharomyces cerevisiae.

Molecular & general genetics : MGG ·Vol. 239 ·No. 1-2 ·1993-05-00 ·Pages 196-204

Kraakman LS, Griffioen G, Zerp S, Groeneveld P, Thevelein JM, Mager WH, Planta RJ

Abstract

The rate of ribosomal protein gene (rp-gene) transcription in yeast is accurately adjusted to the cellular requirement for ribosomes under various growth conditions. However, the molecular mechanisms underlying this co-ordinated transcriptional control have not yet been elucidated. Transcriptional activation of rp-genes is mediated through two different multifunctional transacting factors, ABF1 and RAP1. In this report, we demonstrate that changes in cellular rp-mRNA levels during varying growth conditions are not parallelled by changes in the in vitro binding capacity of ABF1 or RAP1 for their cognate sequences. In addition, the nutritional upshift response of rp-genes observed after addition of glucose to a culture growing on a non-fermentative carbon source turns out not to be the result of increased expression of the ABF1 and RAP1 genes or of elevated DNA-binding activity of these factors. Therefore, growth rate-dependent transcription regulation of rp-genes is most probably not mediated by changes in the efficiency of binding of ABF1 and RAP1 to the upstream activation sites of these genes, but rather through other alterations in the efficiency of transcription activation. Furthermore, we tested the possibility that cAMP may play a role in elevating rp-gene expression during a nutritional shift-up. We found that the nutritional upshift response occurs normally in several mutants defective in cAMP metabolism.

MeSH Terms
Base Sequence Blotting, Northern Cyclic AMP/biosynthesis DNA, Fungal DNA-Binding Proteins/genetics Ethanol/metabolism Fungal Proteins/genetics Gene Expression Regulation, Fungal Genes, Fungal Glucose/metabolism Molecular Sequence Data Repressor Proteins/genetics Ribosomal Proteins/genetics Saccharomyces cerevisiae/genetics,growth & development Saccharomyces cerevisiae Proteins Signal Transduction Telomere-Binding Proteins Transcription Factors Transcription, Genetic
Chemicals
ABF1 protein, S cerevisiae DNA, Fungal DNA-Binding Proteins Fungal Proteins Repressor Proteins Ribosomal Proteins Saccharomyces cerevisiae Proteins Telomere-Binding Proteins Transcription Factors RIF1 protein, S cerevisiae Ethanol Cyclic AMP Glucose
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Kraakman L S
Department of Biochemistry and Molecular Biology, Vrije Universiteit, Amsterdam, The Netherlands.
Griffioen G
Zerp S
Groeneveld P
Thevelein J M
Mager W H
Planta R J
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00281618
Published
1993-05-00
Pages
196-204
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00281618
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