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PMID: 1594441 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Kluyveromyces contains a functional ABF1-homologue.

Nucleic acids research ·Vol. 20 ·No. 9 ·1992-05-11 ·Pages 2211-5

Gonçalves PM, Maurer K, Mager WH, Planta RJ

Abstract

ABF1 is a multifunctional protein present in Saccharomyces cerevisiae, involved in transcription-activation and -repression as well as in DNA-replication. Several lines of evidence indicate the occurrence in the related species Kluyveromyces lactis of a protein having similar properties to those of ABF1 in S. cerevisiae. In order to identify conserved functional domains in ABF1, we have cloned and sequenced the gene encoding the ABF1-homologue from K. lactis. KIABF1 is much smaller than ScABF1 (54.6 vs. 81.7 kD). It exhibits extensive homology with its S. cerevisiae counterpart in the N-terminal region. The C-terminal domain however, is divergent, with the striking exception of a stretch of 20 amino acids, which is virtually identical in the two proteins. KIABF1 can substitute ABF1 in S. cerevisiae, emphasizing the conservation of the multiple functions of this protein.

Related Genes
ABF1
MeSH Terms
Amino Acid Sequence Base Sequence Blotting, Northern Cloning, Molecular DNA, Fungal DNA-Binding Proteins Fungal Proteins/genetics Genes, Fungal Genetic Complementation Test Kluyveromyces/genetics,growth & development Molecular Sequence Data Restriction Mapping Saccharomyces cerevisiae Proteins Sequence Alignment Transcription Factors/genetics
Chemicals
ABF1 protein, S cerevisiae DNA, Fungal DNA-Binding Proteins Fungal Proteins Saccharomyces cerevisiae Proteins Transcription Factors
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Gonçalves P M
Department of Biochemistry and Molecular Biology, Vrije Universiteit, Amsterdam, The Netherlands.
Maurer K
Mager W H
Planta R J
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/20.9.2211
Published
1992-05-11
Pages
2211-5
Language
English
Region
England
NLM ID
0411011
PMCID
PMC312333
Subset
IM
Databases
GENBANK
X64462
External Links
PubMed source DOI: 10.1093/nar/20.9.2211 PMC Full Text View journal details
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