Abstract
In yeast, expression of heat shock genes is regulated by a factor (HSF) which binds constitutively to DNA, but activates transcription efficiently only after heat shock. We have compared the HSFs from Saccharomyces cerevisiae and Kluyveromyces lactis. Both factors contain an activation domain whose activity is masked at low temperature, but the amino acid sequences of these activators are unrelated. Masking requires the evolutionarily conserved DNA binding and oligomerization domains, as well as a short conserved element close to the activator. Although this element contains potential phosphorylation sites, they are not required for induction. We suggest that the conserved element binds either to the structural core of the protein or to another polypeptide, holding the activator in an inactive configuration, and that high temperatures disrupt this interaction. Our results emphasize the importance of global protein structure in the regulation of transcription factor activity.
MeSH Terms
Amino Acid Sequence
Binding Sites
Cloning, Molecular
DNA-Binding Proteins/genetics,metabolism
Genes, Fungal
Heat-Shock Proteins/genetics
Hot Temperature
Kluyveromyces/genetics,metabolism
Macromolecular Substances
Models, Molecular
Molecular Sequence Data
Mutagenesis, Site-Directed
Protein Conformation
Recombinant Fusion Proteins/genetics,metabolism
Saccharomyces cerevisiae/genetics,metabolism
Saccharomyces cerevisiae Proteins
Sequence Homology, Nucleic Acid
Transcription Factors/genetics,metabolism
beta-Galactosidase/genetics,metabolism
Chemicals
DNA-Binding Proteins
HSF1 protein, S cerevisiae
Heat-Shock Proteins
Macromolecular Substances
Recombinant Fusion Proteins
Saccharomyces cerevisiae Proteins
Transcription Factors
beta-Galactosidase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Jakobsen B K
MRC Laboratory of Molecular Biology, Cambridge, UK.
Pelham H R
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