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PMID: 6196722 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Yeast ribosomal protein S33 is encoded by an unsplit gene.

Nucleic acids research ·Vol. 11 ·No. 22 ·1983-11-25 ·Pages 7759-68

Leer RJ, van Raamsdonk-Duin MM, Schoppink PJ, Cornelissen MT, Cohen LH, Mager WH, Planta RJ

Abstract

The structure of the gene coding for ribosomal protein S33, - a protein which escapes the coordinate control of ribosomal protein synthesis in rna 2 mutant cells -, was determined by sequence analysis. The gene comprises an uninterrupted coding region of 204 nucleotides encoding a protein of 8.9 kD. Like for other yeast ribosomal protein genes that have been sequenced so far, a relatively strong codon bias was observed. By S1 nuclease mapping the 5' end of the S33 mRNA was shown to be located at 11 to 15 nucleotides upstream from the initiation codon.

MeSH Terms
Amino Acid Sequence Base Sequence DNA Restriction Enzymes Genes, Fungal Nucleic Acid Hybridization Poly A/genetics Protein Biosynthesis RNA/genetics RNA, Messenger Ribosomal Proteins/genetics Saccharomyces/genetics Saccharomyces cerevisiae/genetics
Chemicals
RNA, Messenger Ribosomal Proteins ribosomal protein S33 Poly A RNA DNA Restriction Enzymes
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Leer R J
van Raamsdonk-Duin M M
Schoppink P J
Cornelissen M T
Cohen L H
Mager W H
Planta R J
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/11.22.7759
Published
1983-11-25
Pages
7759-68
Language
English
Region
England
NLM ID
0411011
PMCID
PMC326528
Subset
IM
Databases
GENBANK
X00128
External Links
PubMed source DOI: 10.1093/nar/11.22.7759 PMC Full Text View journal details
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