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PMID: 355832 Published · ppublish English Journal Article

Yeast ribosomal proteins. I. Characterization of cytoplasmic ribosomal proteins by two-dimensional gel electrophoresis.

Molecular & general genetics : MGG ·Vol. 162 ·No. 3 ·1978-07-04 ·Pages 259-68

Otaka E, Kobata K

Abstract

The cytoplasmic 80s ribosomal proteins from the cells of yeast Sachharomyces cerevisiae were analysed by SDS two-dimensional polyacrylamide gel electrophoresis. Seventyfour proteins were identified and consecutively numbered from 1 to 74. Upon oxidation of the 80s proteins with performic acid, ten proteins (no. 15, 20, 35, 40, 44, 46, 49, 51, 54 and 55) were dislocated on the gel without change of the total number of protein spots. Five proteins (no. 8, 14, 16, 36 and 74) were phosphorylated in vivo as seen in 32P-labelling experiments. The large and small subunits separated in low magnesium medium were analyzed by the above gel electrophoresis. At least forty-five and twenty-eight proteins were assumed to be in the large and small subunits, respectively. All proteins found in the 80s ribosomes, except for no. 3, were detected in either subunit without appearance of new spots. The acidic protein no. 3 seems to be lost during subunit dissociation.

MeSH Terms
Autoradiography Electrophoresis, Polyacrylamide Gel/methods Molecular Weight Ribosomal Proteins/isolation & purification Ribosomes/metabolism Saccharomyces cerevisiae/metabolism Sodium Dodecyl Sulfate
Chemicals
Ribosomal Proteins Sodium Dodecyl Sulfate
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Otaka E
Kobata K
References (36)
36 references, click to expand
  1. The ribosomal proteins of Saccharomyces cerevisiae. Phosphorylated and exchangeable proteins.
    J Biol Chem. 1976 Mar 25;251(6):1799-807 PMID: 767341
  2. Phosphorylation in vitro and in vivo of ribosomal proteins from Saccharomyces cerevisia.
    Eur J Biochem. 1977 Jan 3;72(1):167-74 PMID: 318998
  3. The ribosomal proteins of Escherichia coli. II. Chemical and physical characterization of the 30S ribosomal proteins.
    Biochemistry. 1969 Jul;8(7):2906-15 PMID: 4897207
  4. Determination of the number of proteins in liver ribosomes and ribosomal subunits by two-dimensional polyacrylamide gel electrophoresis.
    J Biol Chem. 1972 Jul 25;247(14):4460-7 PMID: 5043849
  5. Proteins from biologically active ribosomal subparticles of Xenopus leavis.
    Biochim Biophys Acta. 1973 May 17;310(1):188-204 PMID: 4575791
  6. Isolation of eukaryotic ribosomal proteins. Purification and characterization of the 60 S ribosomal subunit proteins L4, L5, L7, L9, L11, L12, L13, L21, L22, L23, L26, L27, L30, L33, L35', L37, and L39.
    J Biol Chem. 1976 Dec 25;251(24):7940-6 PMID: 1002715
  7. Study on proteins from yeast cytoplasmic ribosomes by two-dimensional gel electrophoresis.
    Mol Gen Genet. 1976 Apr 23;145(1):73-9 PMID: 5666
  8. Proteins occurring at, or near, the subunit interface of E. coli ribosomes.
    Mol Gen Genet. 1973 Dec 31;127(4):359-68 PMID: 4594008
  9. Studies on structural proteins of the rat liver ribosomes. I. Molecular wights of the proteins of large and small subunits.
    Biochim Biophys Acta. 1975 Aug 21;402(2):214-29 PMID: 1174538
  10. Coordinate regulation of the synthesis of eukaryotic ribosomal proteins.
    Proc Natl Acad Sci U S A. 1976 May;73(5):1547-51 PMID: 775493
  11. Studies on proteins of animal ribosomes. 13. Enumeration of ribosomal proteins of rat liver.
    FEBS Lett. 1972 Oct 1;26(1):228-32 PMID: 4636733
  12. Molecular-weight determinations and stoichiometric measurements of 40-S and 60-S ribosomal proteins of the cellular slime mold Dictyostelium discoideum.
    Eur J Biochem. 1974 May 15;44(2):469-79 PMID: 4858001
  13. Group fractionation of eukaryotic ribosomal proteins.
    J Biol Chem. 1976 Mar 25;251(6):1808-16 PMID: 1254597
  14. Characterization of the proteins of the small subunits of rat liver ribosomes.
    Biochim Biophys Acta. 1972 Dec 28;285(2):473-82 PMID: 4633644
  15. The number and size of the proteins in the subunits of human placental ribosomes.
    FEBS Lett. 1973 Oct 15;36(2):217-21 PMID: 4754266
  16. Comparison of amino acid sequences among ribosomal proteins of Escherichia coli.
    FEBS Lett. 1976 Jan 1;61(1):14-9 PMID: 1107067
  17. The molecular weights of rat liver ribosomal proteins determined by "three-dimensional" polyacrylamide gel electrophoresis.
    Mol Gen Genet. 1974;134(1):1-6 PMID: 4457752
  18. Further analysis of the protein composition of yeast ribosomes.
    FEBS Lett. 1975 Oct 15;58(1):102-5 PMID: 1225568
  19. Studies on proteins of animal ribosomes. XV. Proteins of the small subunit of rat liver ribosomes: isolation, amino acid composition, tryptic peptides and molecular weights.
    Mol Gen Genet. 1973 Nov 22;126(4):349-56 PMID: 4784812
  20. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.
    J Biol Chem. 1969 Aug 25;244(16):4406-12 PMID: 5806584
  21. Ribosomal proteins from rabbit reticulocytes: number and molecular weights of proteins from ribosomal subunits.
    J Mol Biol. 1975 Apr 15;93(3):391-404 PMID: 49436
  22. The extraction of proteins from eukaryotic ribosomes and ribosomal subunits.
    Mol Gen Genet. 1974;135(2):97-112 PMID: 4457758
  23. Molecular weight distribution of proteins in rabbit reticulocyte ribosomal subunits.
    J Mol Biol. 1971 Oct 14;61(1):143-56 PMID: 5004354
  24. A comparison of the amino-terminal sequence of the L7/L12-type proteins of Artemia salina and Saccharomyces cerevisiae.
    FEBS Lett. 1977 Sep 15;81(2):308-10 PMID: 336394
  25. The ribosomal protein L8 is a complex L7/L12 and L10.
    FEBS Lett. 1976 Apr 15;64(1):135-8 PMID: 773698
  26. Preparation and analysis of structural proteins of the small ribosomal subunit of rat liver.
    Acta Biol Med Ger. 1974;33(5-6):531-7 PMID: 4469371
  27. Isolation of eukaryotic ribosomal proteins. Purification and characterization of 60 S ribosomal subunit proteins L3, L6, L7', L8, L10, L15, L17, L18, L19, L23', L25, L27', L28, L29, L31, L32, L34, L35, L36, L36', and L37'.
    J Biol Chem. 1977 Jun 10;252(11):3961-9 PMID: 863909
  28. Enumeration of rabbit reticulocyte ribosomal proteins.
    J Mol Biol. 1971 Dec 14;62(2):403-5 PMID: 5138339
  29. The ribosomal proteins of Escherichia coli. I. Purification of the 30S ribosomal proteins.
    Biochemistry. 1969 Jul;8(7):2897-905 PMID: 4897206
  30. Ribosomal proteins. 8. Molecular weights of isolated ribosomal proteins of Escherichia coli.
    Proc Natl Acad Sci U S A. 1970 Dec;67(4):1909-13 PMID: 4923120
  31. Two-dimensional polyacrylamide gel electrophoresis: an improved method for ribosomal proteins.
    Anal Biochem. 1974 Jan;57(1):200-10 PMID: 4817496
  32. Methylation of proteins in 60S ribosomal subunits from Saccharomyces cerevisiae.
    FEBS Lett. 1977 Mar 15;75(1):187-91 PMID: 323047
  33. In vivo and in vitro phosphorylation of ribosomal proteins by protein kinases from Saccharomyces cerevisiae.
    Biochemistry. 1976 Jun 1;15(11):2289-96 PMID: 179565
  34. Differences in size and number of 80 S and 70 S ribosomal proteins by dodecyl sulfate gel electrophoresis.
    J Biol Chem. 1971 Nov 25;246(22):6828-34 PMID: 4108251
  35. The stimulation of the phosphorylation of ribosomal protein S6 by cycloheximide and puromycin.
    Biochem Biophys Res Commun. 1974 Oct 23;60(4):1482-90 PMID: 4424298
  36. The isolation of eukaryotic ribosomal proteins. The purification and characterization of the 40 S ribosomal subunit proteins S2, S3, S4, S5, S6, S7, S8, S9, S13, S23/S24, S27, and S28.
    J Biol Chem. 1976 Aug 10;251(15):4666-72 PMID: 947902
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00268851
Published
1978-07-04
Pages
259-68
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00268851
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