Ribosomal proteins. 8. Molecular weights of isolated ribosomal proteins of Escherichia coli.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 67 ·No. 4 ·1970-12-00 ·Pages 1909-13

Dzionara M, Kaltschmidt E, Wittmann HG

Abstract

Molecular weights of the isolated proteins from the 30S and 50S ribosomal subunits of Escherichia coli were determined by two independent methods: polyacrylamide gel electrophoresis with sodium dodecylsulphate and equilibrium sedimentation. The values for the molecular weights determined by gel electrophoresis range from 10,900 to 65,000 for the proteins of the 30S subunit and from 9,600 to 31,500 for those of the 50S subunit, with number averages of 19,000 and 16,300, respectively, in agreement with those obtained by equilibrium sedimentation.

MeSH Terms

Bacterial Proteins/analysis Centrifugation, Density Gradient Chymotrypsin Detergents Electrophoresis, Disc Enzyme Precursors/analysis Escherichia coli/cytology Glyceraldehyde-3-Phosphate Dehydrogenases/analysis Molecular Weight Muramidase/analysis Myoglobin/analysis Ribosomes/analysis Sulfuric Acids

Chemicals

Bacterial Proteins Detergents Enzyme Precursors Myoglobin Sulfuric Acids Glyceraldehyde-3-Phosphate Dehydrogenases Muramidase Chymotrypsin

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Dzionara M

Kaltschmidt E

Wittmann H G

References (13)

13 references, click to expand

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Physical and functional heterogeneity of E. coli ribosomes.
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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.67.4.1909

Published

1970-12-00

Pages

1909-13

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC283446

Subset

External Links

PubMed source DOI: 10.1073/pnas.67.4.1909 PMC Full Text View journal details

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Ribosomal proteins. 8. Molecular weights of isolated ribosomal proteins of Escherichia coli.

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