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PMID: 8380087 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A mouse mammary tumor virus promoter element near the transcription initiation site.

Journal of virology ·Vol. 67 ·No. 1 ·1993-01-00 ·Pages 415-24

Pierce J, Fee BE, Toohey MG, Peterson DO

Abstract

Transcription from the promoter of mouse mammary tumor virus is subject to both positive and negative control by cellular factors, and proviral promoter elements that mediate a basal level of transcription must in some way respond to these cellular regulatory signals. Several such elements, including a TATA box, a region containing three octamer-related sequences, and a binding site for nuclear factor 1, have been previously defined. Additional promoter mutations have allowed a fourth basal promoter element to be identified near the transcription initiation site between +2 and +10. Sequence alterations within this element affect transcription both in vivo and in vitro. Gel electrophoresis mobility shift and DNase I footprinting assays define a nuclear protein, termed initiation site-binding protein, that specifically recognizes this region of the promoter. Optimal levels of transcription from the mouse mammary tumor virus promoter require initiation site-binding protein, as demonstrated by a correlation between protein affinity and transcriptional activity and by specific inhibition of transcription in vitro by an oligonucleotide capable of titrating the protein from transcriptionally active fractions.

MeSH Terms
Animals Base Sequence Binding, Competitive Chromosome Mapping DNA Mutational Analysis DNA-Binding Proteins/metabolism Mammary Tumor Virus, Mouse/genetics Mice Molecular Sequence Data Nuclear Proteins/metabolism Promoter Regions, Genetic/genetics RNA, Viral/biosynthesis Transcription, Genetic/genetics
Chemicals
DNA-Binding Proteins Nuclear Proteins RNA, Viral
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Pierce J
Department of Biochemistry and Biophysics, Texas A&M University, College Station 77843-2128.
Fee B E
Toohey M G
Peterson D O
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.67.1.415-424.1993
Published
1993-01-00
Pages
415-24
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC237378
Subset
IM
Grants
NCI NIH HHS · CA32695 · United States
NCI NIH HHS · CA48041 · United States
External Links
PubMed source DOI: 10.1128/JVI.67.1.415-424.1993 PMC Full Text View journal details
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