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PMID: 3025659 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Multiple hormone-inducible enhancers as mediators of differential transcription.

Molecular and cellular biology ·Vol. 6 ·No. 12 ·1986-12-00 ·Pages 4526-38

Toohey MG, Morley KL, Peterson DO

Abstract

Sets of genes under a common regulatory control in a given cell type are often differentially transcribed. The possibility that this differential transcription can be modulated by the number or strength of cis-acting regulatory sequences associated with a given gene was tested by using the glucocorticoid-responsive enhancer element associated with the mouse mammary tumor virus promoter. Results indicate that differential levels of hormone-inducible gene expression can be modulated in an additive way by the number of glucocorticoid-responsive enhancers associated with this promoter. Realization of these effects shows little preference for position of the additional elements with respect to the promoter. When sequences that bind the glucocorticoid receptor in vitro with somewhat lower affinity than the enhancer were tested, these additive effects were not detected. The results support that differential transcription of genes subject to a common regulatory control can be mediated, at least in part, by the number or strength of their associated cis-acting regulatory sequences.

MeSH Terms
Acetyltransferases/genetics Animals Chloramphenicol O-Acetyltransferase DNA/analysis DNA Restriction Enzymes Dexamethasone/pharmacology Enhancer Elements, Genetic/drug effects Genes, Regulator/drug effects Glucocorticoids/pharmacology L Cells/enzymology Mammary Tumor Virus, Mouse/genetics Mice Plasmids Simian virus 40/genetics Thymidine Kinase/genetics Transcription, Genetic Transfection Tubulin/genetics
Chemicals
Glucocorticoids Tubulin Dexamethasone DNA Acetyltransferases Chloramphenicol O-Acetyltransferase Thymidine Kinase DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Toohey M G
Morley K L
Peterson D O
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.6.12.4526-4538.1986
Published
1986-12-00
Pages
4526-38
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC367237
Subset
IM
Grants
NCI NIH HHS · CA32695 · United States
External Links
PubMed source DOI: 10.1128/mcb.6.12.4526-4538.1986 PMC Full Text View journal details
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