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PMID: 2838741 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Simian virus 40 major late promoter: a novel tripartite structure that includes intragenic sequences.

Molecular and cellular biology ·Vol. 8 ·No. 5 ·1988-05-00 ·Pages 2021-33

Ayer DE, Dynan WS

Abstract

Unlike most genes transcribed by RNA polymerase II, the simian virus 40 late transcription unit does not have a TATA box. To determine what sequences are required for initiation at the major late mRNA cap site of simian virus 40, clustered point mutations were constructed and tested for transcriptional activity in vitro and in vivo. Three promoter elements were defined. The first is centered 31 base pairs upstream of the cap site in a position normally reserved for a TATA box. The second is at the cap site. The third occupies a novel position centered 28 base pairs downstream of the cap site within a protein-coding sequence. The ability of RNA polymerase II to recognize this promoter suggests that there is greater variation in promoter architecture than had been believed previously.

MeSH Terms
Base Sequence Genes, Viral Molecular Sequence Data Promoter Regions, Genetic RNA Caps RNA, Viral/genetics Simian virus 40/genetics Transcription, Genetic
Chemicals
RNA Caps RNA, Viral
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Ayer D E
Department of Chemistry and Biochemistry, University of Colorado, Boulder 80309-0215.
Dynan W S
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.8.5.2021-2033.1988
Published
1988-05-00
Pages
2021-33
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC363381
Subset
IM
Grants
NIGMS NIH HHS · GM 35866 · United States
NCRR NIH HHS · RR 07013 · United States
External Links
PubMed source DOI: 10.1128/mcb.8.5.2021-2033.1988 PMC Full Text View journal details
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