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PMID: 3025848 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Elevated levels of mRNA can account for the trans-activation of human immunodeficiency virus.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 24 ·1986-12-00 ·Pages 9734-8

Peterlin BM, Luciw PA, Barr PJ, Walker MD

Abstract

The genome of human immunodeficiency virus encodes a protein that dramatically elevates amounts of viral proteins. The precise mechanism of this trans-activation remains to be established. It has been reported that trans-activation can occur without major changes in the levels of mRNA. We constructed recombinant plasmids containing those viral sequences required in cis for trans-activation linked to the chloramphenicol acetyltransferase gene. These plasmids were introduced into cultured cells in either the presence or absence of a second plasmid that directed expression of the viral trans-activator protein. Expression of the chloramphenicol acetyltransferase gene was measured at the level of protein (by enzymatic assay) and RNA (by ribonuclease protection and primer extension). Our results demonstrate that trans-activation is accompanied by large increases in mRNA levels; these increases may be sufficient to explain the elevated levels of trans-activated protein.

MeSH Terms
Deltaretrovirus/genetics Enhancer Elements, Genetic Gene Expression Regulation Genes, Regulator Genes, Viral Promoter Regions, Genetic RNA, Messenger/genetics Transcription, Genetic Virus Replication
Chemicals
RNA, Messenger
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Peterlin B M
Luciw P A
Barr P J
Walker M D
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47 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.24.9734
Published
1986-12-00
Pages
9734-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC387215
Subset
IM
Grants
NIGMS NIH HHS · GM 28520 · United States
External Links
PubMed source DOI: 10.1073/pnas.83.24.9734 PMC Full Text View journal details
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