Insulin synthesis in a clonal cell line of simian virus 40-transformed hamster pancreatic beta cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 78 ·No. 7 ·1981-07-00 ·Pages 4339-43

Santerre RF, Cook RA, Crisel RM, Sharp JD, Schmidt RJ, Williams DC, Wilson CP

Abstract

A clonal hamster beta cell line (HIT) was established by simian virus 40 transformation of Syrian hamster pancreatic islet cells. Cytoplasmic insulin was detected in all cells by indirect fluorescent antibody staining, and membrane-bound secretory granules were observed ultrastructurally. Acidified-ethanol extracts of HIT cell cultures contained hamster insulin as determined by radioimmunoassay, radioreceptor assay, and bioassay. One subclone at passage 39 contained 2.6 micrograms of insulin per mg of cell protein. [3H]Leucine-labeled HIT insulin and proinsulin were identical to islet-derived proteins when compared by NaDodSO4/polyacrylamide gel electrophoresis of immunoprecipitates. HIT cell insulin secretion was stimulated by glucose, glucagon, and 3-isobutyl-1-methylxanthine. Insulin secretion at optimal glucose concentration (7.5 mM) was 2.4 milliunits per 10(6) cells per hr. Somatostatin and dexamethasone markedly inhibited HIT insulin secretion. The HIT cell line represents a unique in vitro system for studying beta cell metabolism and insulin biosynthesis.

MeSH Terms

Animals Cell Line Cell Transformation, Viral Clone Cells Cricetinae Glucagon/pharmacology Glucose/pharmacology Insulin/biosynthesis,metabolism Insulin Secretion Islets of Langerhans/metabolism Mesocricetus Secretory Rate/drug effects Simian virus 40

Chemicals

Insulin Glucagon Glucose

Authors & Affiliations

7 authors, click to expand affiliations / ORCID

Santerre R F

Cook R A

Crisel R M

Sharp J D

Schmidt R J

Williams D C

Wilson C P

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.78.7.4339

Published

1981-07-00

Pages

4339-43

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC319785

Subset

External Links

PubMed source DOI: 10.1073/pnas.78.7.4339 PMC Full Text View journal details

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Insulin synthesis in a clonal cell line of simian virus 40-transformed hamster pancreatic beta cells.

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