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PMID: 231763 Published · ppublish English Journal Article

Characterization of the major altered leader sequence of late mRNA induced by SV40 deletion mutant d1-1811.

Nucleic acids research ·Vol. 7 ·No. 7 ·1979-12-11 ·Pages 1799-814

Haegeman G, Iserentant D, Gheysen D, Fiers W

Abstract

d1-1811 is a viable SV40 mutant with a 40 base pair deletion that includes the major wild-type capping site of late mRNA at map position 0.72. The late viral mRNAs induced by d1-1811 have now been further characterized by inversed S1-mapping analysis. The S1-resistant, 32P-labeled RNA fragments derived from the leader region were examined by fingerprinting and by analysis of RNase T2-generated 5'-terminal cap structures. The results show that most if not all of the mutant leader fragments analyzed have their 5' terminus to the left of the d1-1811 deletion site, i.e., closer to the origin of DNA replication. The major altered leader fragment is a continuous transcript from the DNA in the region 0.716 to 0.761 map unit and its 5' terminus has been precisely mapped at nucleotide L290. The observation that the cap structure of the major altered leader is only a minor cap species in wild-type late RNA suggests regulation in the use of different capping sites in SV40.

MeSH Terms
Base Sequence Chromosome Deletion Chromosome Mapping Electrophoresis, Polyacrylamide Gel Mutation Nucleic Acid Conformation RNA Caps/genetics RNA, Messenger/genetics Simian virus 40/genetics
Chemicals
RNA Caps RNA, Messenger
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Haegeman G
Iserentant D
Gheysen D
Fiers W
References (36)
36 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/7.7.1799
Published
1979-12-11
Pages
1799-814
Language
English
Region
England
NLM ID
0411011
PMCID
PMC342349
Subset
IM
Databases
GENBANK
J02400, J02402, J02403, J02406, J02407, J02408, J02409, J02410, V01380
External Links
PubMed source DOI: 10.1093/nar/7.7.1799 PMC Full Text View journal details
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