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PMID: 205672 Published · ppublish English Journal Article

Characterization of the 5'-terminal capped structures of late simian virus 40-specific mRNA.

Journal of virology ·Vol. 25 ·No. 3 ·1978-03-00 ·Pages 824-30

Haegeman G, Fiers W

Abstract

32P-labeled, late simian virus 40-specific RNA was isoalted from infected CV1 cells and completely degraded with RNase T2 and bacterial alkaline phosphatase. The RNase-resistant material was fractionated two dimensionally and further characterized with Penicillium nuclease and nucleotide pyrophosphatase. Two major 5' termini were identified in late simian virus 40 RNA, namely, 7-methyl Gppp 2',6-dimethyl ApUp and 7-methyl Gppp 2',6-dimethyl Ap 2'-methyl, UpUp. Both 5' termini are present in unfractionated viral RNA as well as in the separated 16S and 19S species. As both caps differ only in secondary modification, it is possible that they are derived from the same site on the DNA. The relatively higher cap II content of the 16S mRNA may be related to its slower rate of turnover.

MeSH Terms
Base Sequence Cell Line Coliphages/enzymology Guanine Nucleotides/analysis Hydrolysis Methylation Oligonucleotides/analysis RNA, Messenger/analysis RNA, Viral/analysis Ribonucleases/metabolism Simian virus 40/analysis
Chemicals
Guanine Nucleotides Oligonucleotides RNA, Messenger RNA, Viral Ribonucleases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Haegeman G
Fiers W
References (33)
33 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.25.3.824-830.1978
Published
1978-03-00
Pages
824-30
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC525976
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.25.3.824-830.1978 PMC Full Text View journal details
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