The methylation of adenovirus-specific nuclear and cytoplasmic RNA.

Nucleic acids research ·Vol. 3 ·No. 3 ·1976-03-00 ·Pages 749-65

Sommer S, Salditt-Georgieff M, Bachenheimer S, Darnell JE, Furuichi Y, Morgan M, Shatkin AJ

Abstract

Each poly(A) containing cytoplasmic AD-2 MRNA contains at its 5' terminus the general structure m7 GpppN1 pN2p or m7 GpppN1mpN2mpNp as well as an average of 4 m6A and 0.5-1 m5C residues per molecule. Almost all of the N1m residues are adenine derivatives including Am, m6Am and probably m26,6Am. The N2m is mostly Cm but small amounts of the other three methylated bases are also present. All the methylated constitutents of mRNA are distant from the 3' terminal poly(A). The amount of m6A appears to be greater in larger mRNA than in smaller mRNA. Nuclear Ad-2 specific RNA also contains caps, m6A, and m5C with about twice as much m6A relative to caps as cytoplasmic mRNA. The similarity of Ad-2 nuclear and mRNA to HeLa hnRNA and mRNA suggests that adenovirus mRNA production is a good model for eukaryotic mRNA production.

MeSH Terms

Adenoviridae/metabolism Cell Nucleus/metabolism Cytoplasm/metabolism HeLa Cells/metabolism Nucleic Acid Hybridization Poly U/analysis RNA, Viral/metabolism Ribonucleases tRNA Methyltransferases/metabolism

Chemicals

RNA, Viral Poly U tRNA Methyltransferases Ribonucleases

Authors & Affiliations

7 authors, click to expand affiliations / ORCID

Sommer S

Salditt-Georgieff M

Bachenheimer S

Darnell J E

Furuichi Y

Morgan M

Shatkin A J

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/3.3.749

Published

1976-03-00

Pages

749-65

Language

English

Region

England

NLM ID

0411011

PMCID

PMC342938

Subset

External Links

PubMed source DOI: 10.1093/nar/3.3.749 PMC Full Text View journal details

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The methylation of adenovirus-specific nuclear and cytoplasmic RNA.

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