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PMID: 170607 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

RNAs of simian virus 40 in productively infected monkey cells: kinetics of formation and decay in enucleate cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 72 ·No. 7 ·1975-07-00 ·Pages 2587-91

Aloni Y, Shani M, Reuveni Y

Abstract

We demonstrate here the usefulness of cytochalasin B enucleate cells for the study of the metabolism of cytoplasmic mRNA and for determining its half-life in animal cells. Simian virus 40 infected monkey cells in which the RNA had been labeled with [3H]uridine were enucleated, and the decay of the two prominent RNAs of simian virus 40, the 19S and 16S species, was measured by analysis on sucrose gradients. The results of these experiments, together with kinetic analysis of nuclear and cytoplasmic viral RNA, indicate a precursor-product relationship between the 19S and 16S cytoplasmic viral RNA species, which decay by first-order kinetics with a mean half-life of about 3 hr and 6 hr, respectively.

MeSH Terms
Animals Cell Line Cell Nucleus/drug effects,metabolism Cytochalasin B/pharmacology Haplorhini Kinetics RNA, Viral/metabolism Simian virus 40/drug effects,metabolism Time Factors Transcription, Genetic/drug effects
Chemicals
RNA, Viral Cytochalasin B
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Aloni Y
Shani M
Reuveni Y
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25 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.72.7.2587
Published
1975-07-00
Pages
2587-91
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC432814
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.72.7.2587 PMC Full Text View journal details
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