Synthesis of infective poliovirus in BSC-1 monkey cells enucleated with cytochalasin B.

Science (New York, N.Y.) ·Vol. 179 ·No. 4076 ·1973-03-02 ·Pages 915-6

Pollack R, Goldman R

Abstract

BSC-1 monkey kidney cells were enucleated by two cycles of centrifugation in the presence of cytochalasin B. The resulting populations contained 99.5 percent enucleated cells. After infection, newly synthesized poliovirus was recovered from the enucleated populations. Final virus yield per enucleated cell was about one-fifth the yield per infected untreated cell

MeSH Terms

Animals Antigens, Viral/analysis Cell Fractionation Cell Line Cell Nucleus/drug effects Cells, Cultured Cytochalasin B/pharmacology Cytoplasm/immunology Haplorhini Indoles/pharmacology Kidney Microscopy, Fluorescence Mitosporic Fungi Poliovirus/growth & development,immunology Viral Plaque Assay Virus Replication/drug effects

Chemicals

Antigens, Viral Indoles Cytochalasin B

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Pollack R

Goldman R

Article Info

Journal

Science (New York, N.Y.)

Abbr.

Science

ISSN

0036-8075

DOI

10.1126/science.179.4076.915

Published

1973-03-02

Pages

915-6

Language

English

Region

United States

NLM ID

0404511

Subset

External Links

PubMed source DOI: 10.1126/science.179.4076.915 View journal details

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Synthesis of infective poliovirus in BSC-1 monkey cells enucleated with cytochalasin B.

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