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PMID: 202950 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

5'-Terminal sequences and coding region of late simian virus 40 mRNAs are derived from noncontiguous segments of the viral genome.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 74 ·No. 12 ·1977-12-00 ·Pages 5323-7

Lavi S, Groner Y

Abstract

The region of the simian virus 40 genome complementary to the 5' end of the most abundant poly(A)-containing 19S and 16S mRNAs was mapped by hybridization of double-labeled RNA ([3H]methyl group and [14C]uridine) to specific DNA fragments. Chemical identification of methylated residues indicated that a common "leader" sequence adjacent to the 5' terminus of both 19S and 16S mRNA is transcribed from DNA sequences located between 0.67 and 0.76 map units. The estimated size of this "leader" RNA, which does not code for any known viral protein, is 170-200 nucleotides. Our results indicate that sequences complementary to the "leader" region and coding portion of 16S mRNA are located in separate parts of the simian virus 40 genome.

MeSH Terms
Base Sequence Chromosome Mapping DNA, Viral/genetics Guanine Nucleotides/metabolism Methylation Molecular Weight Nucleic Acid Hybridization RNA, Messenger/genetics RNA, Viral/genetics Simian virus 40/genetics
Chemicals
DNA, Viral Guanine Nucleotides RNA, Messenger RNA, Viral
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Lavi S
Groner Y
References (15)
15 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.74.12.5323
Published
1977-12-00
Pages
5323-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC431702
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.74.12.5323 PMC Full Text View journal details
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