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PMID: 2942764 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Control of adenovirus late promoter expression in two human cell lines.

Molecular and cellular biology ·Vol. 5 ·No. 9 ·1985-09-00 ·Pages 2433-42

Lewis ED, Manley JL

Abstract

We investigated the nucleotide sequence requirements of the adenovirus 2 late promoter when activated by either a trans-acting regulatory protein or a cis-acting enhancer element. Using deletion mutants in transient expression assays, we determined that the 5' limit of the region required for activation by a trans-acting regulatory protein, the adenovirus early region 1a gene product, and the simian virus 40 enhancer is the same in both 293 and HeLa cells. Surprisingly, the 3' limit of required sequences varied, depending on the mechanism of activation. Activation mediated by the early region 1a protein endogenous in 293 cells or produced after cotransfection of HeLa cells requires the region around the transcriptional start site, whereas activation brought about by an enhancer element in HeLa cells has no requirement for these sequences. Under no conditions tested did the simian virus 40 enhancer activate the late promoter in 293 cells, even when sequences sufficient for enhancer-mediated activation in HeLa cells, but not for early region 1a activation, were present. These results suggest the existence of at least two different mechanisms for positive regulation of promoter activity.

MeSH Terms
Adenovirus Early Proteins Adenoviruses, Human/genetics Base Sequence Cell Line Enhancer Elements, Genetic Gene Expression Regulation Genes, Viral HeLa Cells Humans Oncogene Proteins, Viral/physiology Promoter Regions, Genetic Transcription, Genetic
Chemicals
Adenovirus Early Proteins Oncogene Proteins, Viral
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Lewis E D
Manley J L
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62 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.5.9.2433-2442.1985
Published
1985-09-00
Pages
2433-42
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC366971
Subset
IM
Grants
PHS HHS · 28983 · United States
Databases
GENBANK
M12460
External Links
PubMed source DOI: 10.1128/mcb.5.9.2433-2442.1985 PMC Full Text View journal details
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