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PMID: 6306563 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

S1 sensitive sites in adenovirus DNA.

Nucleic acids research ·Vol. 11 ·No. 1 ·1983-01-11 ·Pages 21-36

Goding CR, Russell WC

Abstract

S1 nuclease has been used as a probe for regions of DNA secondary structure in supercoiled recombinant plasmids containing adenovirus (Ad) DNA sequences. In the sequences examined two S1 sensitive sites were identified in the left-terminal 16.5% of Ad 12 DNA, one of which aligned approximately with an inverted repeat region in the DNA sequence. In addition an S1 sensitive site was dictated by a potential cruciform structure in the region of the Ad 2 major late promoter. In contrast to the expected cleavage site at the loop of the cruciform, cleavage occurred at the base of the stem in the region of the TATA box. All three S1 sensitive sites identified were more sensitive to S1 than the endogenous sites in the parent plasmids.

MeSH Terms
Adenoviridae/genetics DNA Restriction Enzymes DNA, Viral/genetics Endonucleases/metabolism Nucleic Acid Conformation Nucleic Acid Hybridization Operon Plasmids Single-Strand Specific DNA and RNA Endonucleases
Chemicals
DNA, Viral Endonucleases DNA Restriction Enzymes Single-Strand Specific DNA and RNA Endonucleases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Goding C R
Russell W C
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31 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/11.1.21
Published
1983-01-11
Pages
21-36
Language
English
Region
England
NLM ID
0411011
PMCID
PMC325688
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/11.1.21 PMC Full Text View journal details
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