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PMID: 1702540 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Drosophila retrotransposon promoter includes an essential sequence at the initiation site and requires a downstream sequence for full activity.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 88 ·No. 1 ·1991-01-01 ·Pages 102-4

Jarrell KA, Meselson M

Abstract

We describe a 98-base-pair region (-38 to +60) in the long terminal repeat of the Drosophila gypsy retrotransposon that is sufficient for accurate normal-level transcription. We find that, unlike most RNA polymerase II (pol II) promoters, the gypsy promoter includes downstream sequences that are required for full activity. Also unlike most pol II promoters, the gypsy promoter, which lacks a TATA motif, was found to have an essential sequence at the transcription initiation site, mutation of which abolishes transcription. These three uncommon features of the gypsy promoter may be characteristic of a subset of pol II promoters, exemplified by certain retrotransposons and developmental genes of Drosophila and by Tdt, the mouse terminal deoxynucleotidyl-transferase (TdT) gene.

MeSH Terms

Animals Base Sequence DNA Transposable Elements Drosophila/genetics Molecular Sequence Data Plasmids Poly A/genetics,isolation & purification Promoter Regions, Genetic RNA/genetics,isolation & purification RNA Polymerase II/genetics RNA, Messenger Transcription, Genetic Transfection

Chemicals

DNA Transposable Elements RNA, Messenger Poly A RNA RNA Polymerase II

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Jarrell K A

Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, MA 02138.

Meselson M

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21 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.88.1.102

Published

1991-01-01

Pages

102-4

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC50757

Subset

Databases

GENBANK

M63280, M80719, M80720, M80721, M80722, M80723, M80724, S66292, S66294, S66374

External Links

PubMed source DOI: 10.1073/pnas.88.1.102 PMC Full Text View journal details

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Drosophila retrotransposon promoter includes an essential sequence at the initiation site and requires a downstream sequence for full activity.

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