Suppression in Drosophila: su(Hw) and su(f) gene products interact with a region of gypsy (mdg4) regulating its transcriptional activity.

The EMBO journal ·Vol. 8 ·No. 3 ·1989-03-00 ·Pages 903-11

Mazo AM, Mizrokhi LJ, Karavanov AA, Sedkov YA, Krichevskaja AA, Ilyin YV

Abstract

The gypsy (mdg4) mobile element of Drosophila contains two closely spaced regions which bind proteins from nuclear extracts. One of these is an imperfect palindrome having homology with the lac-operator of Escherichia coli; the other contains a reiterated sequence (5'PyPuT/C TGCATAC/TPyPy) homologous to the octamer that is the core of many enhancers and upstream promoter elements. Transient expression of deletion mutants has shown that these DNA regions are negative and positive regulators of transcription. As was demonstrated earlier by other authors, mutations induced by the presence of gypsy in different loci are suppressed owing to either repression or activation of gypsy transcription in Drosophila strains carrying unlinked mutations in su(Hw) or su(f) genes. We have shown that binding to a negative regulator (silencer) is weakened in nuclear extracts isolated from fly stocks carrying su(f) mutations which activate gypsy transcription; therefore the su(f) gene seems to code for a protein capable of gypsy repression. Furthermore, binding to a positive regulator is weakened in nuclear extracts isolated from fly stocks carrying su(Hw) gene mutations which decrease the level of gypsy transcription; therefore, the su(Hw) gene most likely encodes a protein which activates gypsy transcription.

MeSH Terms

Animals Base Sequence Chromosome Deletion DNA/genetics Drosophila melanogaster/genetics,metabolism Genes, Regulator Molecular Sequence Data Mutation Nuclear Proteins/metabolism Repetitive Sequences, Nucleic Acid Suppression, Genetic Transcription Factors/genetics,metabolism Transcription, Genetic

Chemicals

Nuclear Proteins Transcription Factors DNA

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Mazo A M

Engelhardt Institute of Molecular Biology, USSR Academy of Science, Moscow.

Mizrokhi L J

Karavanov A A

Sedkov Y A

Krichevskaja A A

Ilyin Y V

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34 references, click to expand

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Article Info

Journal

The EMBO journal

Abbr.

EMBO J

ISSN

0261-4189

Published

1989-03-00

Pages

903-11

Language

English

Region

England

NLM ID

8208664

PMCID

PMC400890

Subset

External Links

PubMed source PMC Full Text View journal details

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Suppression in Drosophila: su(Hw) and su(f) gene products interact with a region of gypsy (mdg4) regulating its transcriptional activity.

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