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PMID: 8225598 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Expression of the Salmonella virulence plasmid gene spvB in cultured macrophages and nonphagocytic cells.

Infection and immunity ·Vol. 61 ·No. 12 ·1993-12-00 ·Pages 5231-6

Fierer J, Eckmann L, Fang F, Pfeifer C, Finlay BB, Guiney D

Abstract

Certain serotypes of salmonellae carry virulence plasmids that greatly enhance the pathogenicity of these bacteria in experimentally infected mice. This phenotype is largely attributable to the 8-kb spv regulon. However, spv genes are not expressed while bacteria grow in vitro. We now show that spvB, which is required for virulence, is expressed rapidly after Salmonella dublin is ingested by cultured J774 and murine peritoneal macrophages and that expression is not affected by the alkalinization of intracellular vesicles. The level of induction of spvB is reduced when macrophages are pretreated with gamma interferon. spvB is also expressed in human and canine epithelial cell lines and a human hepatoma cell line. In all cases, spvB expression is dependent on the spvR gene, just as it is in stationary-phase cultures in vitro. These data suggest that spv virulence genes are expressed by intracellular salmonellae in vivo in response to a signal that is common to the intracellular compartments of cells that are invaded by salmonellae.

Related Genes
lacZ spvB
MeSH Terms
Animals Cells, Cultured Dogs Epithelium/microbiology Gene Expression Genes, Bacterial Humans Lac Operon Liver/microbiology Macrophages/microbiology Mice Plasmids Salmonella/genetics,pathogenicity Virulence/genetics
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Fierer J
Department of Veterans Affairs Medical Center, San Diego, California 92161.
Eckmann L
Fang F
Pfeifer C
Finlay B B
Guiney D
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.61.12.5231-5236.1993
Published
1993-12-00
Pages
5231-6
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC281306
Subset
IM
Grants
NIDDK NIH HHS · DK01408 · United States
NIDDK NIH HHS · DK35108 · United States
External Links
PubMed source DOI: 10.1128/iai.61.12.5231-5236.1993 PMC Full Text View journal details
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